Molecular interferometric imaging process and apparatus
First Claim
1. A molecular interferometric imaging system for detecting an analyte in a sample, the molecular interferometric imaging system comprising:
- an illumination source providing a beam of radiation;
a pixel array comprising a plurality of pixels for detecting radiation at a plurality of locations in an image plane to produce an image comprising a plurality of pixel readings;
a biolayer designed to react to the analyte when the biolayer comes in contact with the sample;
a substrate, the biolayer being located on the substrate, the substrate being designed to convert phase modulation into intensity modulation which can be detected and imaged directly by the pixel array;
a reference surface;
an image switching means for switching between a first position in which the beam provided by the illumination source is incident on the biolayer and a reflected sample beam is produced which is directed to the pixel array to generate a sample image, and a second position in which the beam provided by the illumination source is incident on the reference surface and a reflected reference beam is produced which is directed to the pixel array to generate a reference image; and
a processing means for producing a composite image using the sample image and the reference image for illumination normalization.
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Abstract
A molecular interferometric imaging system for detecting an analyte in a sample, that includes an illumination source providing a beam of radiation; a pixel array for detecting radiation in an image plane; a biolayer designed to react to the analyte when it comes in contact with the sample; a substrate designed to convert phase modulation into intensity modulation which can be detected and imaged directly by the pixel array, the biolayer being on the substrate; a reference surface; an image switching means for switching between a first position for collecting a sample image of the biolayer, and a second position for collecting a reference image of the reference surface; and a processing means for producing a composite image using the sample image and the reference image for illumination normalization.
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Citations
34 Claims
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1. A molecular interferometric imaging system for detecting an analyte in a sample, the molecular interferometric imaging system comprising:
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an illumination source providing a beam of radiation; a pixel array comprising a plurality of pixels for detecting radiation at a plurality of locations in an image plane to produce an image comprising a plurality of pixel readings; a biolayer designed to react to the analyte when the biolayer comes in contact with the sample; a substrate, the biolayer being located on the substrate, the substrate being designed to convert phase modulation into intensity modulation which can be detected and imaged directly by the pixel array; a reference surface; an image switching means for switching between a first position in which the beam provided by the illumination source is incident on the biolayer and a reflected sample beam is produced which is directed to the pixel array to generate a sample image, and a second position in which the beam provided by the illumination source is incident on the reference surface and a reflected reference beam is produced which is directed to the pixel array to generate a reference image; and a processing means for producing a composite image using the sample image and the reference image for illumination normalization. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. A method of molecular interferometric imaging for detecting an analyte in a sample using a pixel array comprising a plurality of pixels for detecting radiation at a plurality of locations in an image plane, and a substrate designed to convert phase modulation into intensity modulation which can be detected and imaged directly by the pixel array, the method comprising:
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exposing a biolayer that is deposited on the substrate to the sample, the biolayer being designed to react to the analyte when the biolayer comes in contact with the sample; positioning an image switching means in a first position; illuminating the biolayer with a radiation source to produce a sample beam; capturing the sample beam in a sample image using the pixel array; positioning the image switching means in a second position; illuminating a reference surface with the radiation source to produce a reference beam; capturing the reference beam in a reference image using the pixel array; computing a composite image using the sample image and the reference image for illumination normalization. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification