Multivalent immunoglobulin-based bioactive assemblies
First Claim
1. A hexameric stably tethered structure comprising an IgG antibody attached to two AD2 moieties of SEQ ID NO:
- 4 and four antigen-binding antibody fragments of the same or different IgG with each fragment attached to a DDD2 moiety of SEQ ID NO;
2;
the AD2 moieties bound to the DDD2 moieties.
1 Assignment
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Accused Products
Abstract
The present invention concerns methods and compositions for stably tethered structures of defined compositions, which may have multiple functionalities and/or binding specificities. Preferred embodiments concern hexameric stably tethered structures comprising one or more IgG antibody fragments and which may be monospecific or bispecific. The disclosed methods and compositions provide a facile and general way to obtain stably tethered structures of virtually any functionality and/or binding specificity. The stably tethered structures may be administered to subjects for diagnostic and/or therapeutic use, for example for treatment of cancer or autoimmune disease. The stably tethered structures may bind to and/or be conjugated to a variety of known effectors, such as drugs, enzymes, radionuclides, therapeutic agents and/or diagnostic agents.
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Citations
23 Claims
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1. A hexameric stably tethered structure comprising an IgG antibody attached to two AD2 moieties of SEQ ID NO:
- 4 and four antigen-binding antibody fragments of the same or different IgG with each fragment attached to a DDD2 moiety of SEQ ID NO;
2;
the AD2 moieties bound to the DDD2 moieties. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- 4 and four antigen-binding antibody fragments of the same or different IgG with each fragment attached to a DDD2 moiety of SEQ ID NO;
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11. A hexameric stably tethered structure comprising an JgG antibody attached at its C-terminal end to two AD2 moieties of SEQ ID NO:
- 4and four Fab fragments of the same or different IgG antibody with each Fab fragment attached at its C-terminal end to a DDD2 moiety of SEQ ID NO;
2 the AD2 moieties bound to the DDD2 moieties. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- 4and four Fab fragments of the same or different IgG antibody with each Fab fragment attached at its C-terminal end to a DDD2 moiety of SEQ ID NO;
Specification