Process for producing ethyl alcohol from cellulosic materials
First Claim
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1. A method for producing ethanol from cellulose, the method comprising the steps of:
- a) inoculating an aqueous mixture comprising said cellulose with a first consortium of ethanologenic microorganisms comprising bacteria, fungi or mixtures thereof, and said first consortium comprising at least one bacteria species which uses a carbon source as its electron donor and an electron receptor which is Fe+3, said bacteria species expresses enzymes which hydrolyze said cellulose to produce fermentable sugars; and
b) inoculating said aqueous mixture with a second consortium of microorganisms comprising at least one species which is capable of fermenting said fermentable sugars produced from step a);
wherein the fermentable sugars are produced without using acid hydrolysis and without using additional enzymes.
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Abstract
A method for producing ethanol from cellulose, the method including the steps of hydrolyzing said cellulose with enzymes produced by ethanologenic microorganisms to form a fermentable sugar or mixture of fermentable sugars, and fermenting the fermentable sugars with a yeast consortium to produce ethanol.
32 Citations
22 Claims
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1. A method for producing ethanol from cellulose, the method comprising the steps of:
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a) inoculating an aqueous mixture comprising said cellulose with a first consortium of ethanologenic microorganisms comprising bacteria, fungi or mixtures thereof, and said first consortium comprising at least one bacteria species which uses a carbon source as its electron donor and an electron receptor which is Fe+3, said bacteria species expresses enzymes which hydrolyze said cellulose to produce fermentable sugars; and b) inoculating said aqueous mixture with a second consortium of microorganisms comprising at least one species which is capable of fermenting said fermentable sugars produced from step a);
wherein the fermentable sugars are produced without using acid hydrolysis and without using additional enzymes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for producing fermentable sugars, the method comprising the steps of:
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a) providing a cellulose; b) adding to said source of cellulose a first consortium of ethanologenic microorganisms, said consortium comprising at least one bacteria species which uses carbon as an electron donor and Fe+3 as an electron acceptor wherein said microorganisms produce enzymes sufficient for enzymatic hydrolysis of said cellulose to fermentable sugars;
wherein the fermentable sugars are produced without using acid hydrolysis and without using additional enzymes. - View Dependent Claims (19, 20, 21)
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22. A method for producing ethanol from cellulose, the method comprising the steps of:
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a) inoculating an aqueous mixture comprising said cellulose with a first consortium of ethanologenic microorganisms comprising bacteria, fungi or mixtures thereof, and said first consortium comprising at least one bacteria species which is Enterobacter Cloacae, which expresses enzymes which hydrolyze said cellulose to produce fermentable sugars; b) inoculating said aqueous mixture with a second consortium of microorganisms comprising at least one species which is capable of fermenting said fermentable sugars produced from step a).
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Specification