Engineering fungi for the utilisation of L-arabinose

• US 7,527,951 B2
• Filed: 02/15/2002
• Issued: 05/05/2009
• Est. Priority Date: 02/16/2001
• Status: Expired due to Fees

• Current Assignee: Valtion Teknillinen Tutkimuskeskus
• Original Assignee: Valtion Teknillinen Tutkimuskeskus
• Inventors: Richard, Peter, Londesborough, John, Penttila, Merja
• Primary Examiner(s): Zeman; Robert A
• Application Number:
  US10/257,821
• Publication Number:
  US 20030186402A1
• Time in Patent Office: 2,636 Days
• Field of Search: None
• US Class Current: 435/171
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12Y 101/0101 L-Xylulose reductase (1.1.1.10)
• CPC Class Codes: C12Y 101/01012 L-Arabinitol 4-dehydrogenase (1.1.1.12)
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

Claims

1. A method of producing a useful product from biomass containing L-arabinose, which comprises:

(a) providing a genetically modified fungus which is transformed with a DNA encoding an enzyme selected from the group consisting of L-arabinitol 4-dehydrogenase (LA4D) and L-xylulose reductase (LXR), each of the fungal L-arabinose pathway, wherein said fungus has an increased ability to utilize L-arabinose; and

(b) fermenting said fungus under conditions suitable to produce said useful product,

wherein the DNA is selected from the group consisting of SEQ ID NO. 1, and SEQ ID NO. 2.