Utility of phylloplanins as antibiotics, selective fungicides and for enhancing microbial resistance in crop plants

• US 7,528,232 B2
• Filed: 12/15/2005
• Issued: 05/05/2009
• Est. Priority Date: 05/20/2005
• Status: Active Grant

• Current Assignee: University Of Kentucky Research Foundation (University of Kentucky)
• Original Assignee: University Of Kentucky Research Foundation (University of Kentucky)
• Inventors: Shepherd, Ryan W., Wagner, George J.
• Primary Examiner(s): Desai; Anand U
• Application Number:
  US11/304,528
• Publication Number:
  US 20070006341A1
• Time in Patent Office: 1,237 Days
• Field of Search: None
• US Class Current: 530/350
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8222 Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation

Abstract

The invention is directed to methods and compositions that inhibit pathogen proliferation. More particularly, these methods and compositions involve the use of phylloplane proteins as antibiotics against human and animal pathogens.

Claims

1. An isolated polypeptide comprising a sequence that is at least 95% identical to SEQ ID NO:

18, wherein the polypeptide is a disease- or pest-resistance conferring protein.

2. The isolated polypeptide of claim 1, wherein the polypeptide comprises a sequence that is at least 98% identical to SEQ ID NO:

18, wherein the polypeptide is a disease- or pest-resistance conferring protein.

3. The isolated polypeptide of claim 1, wherein the polypeptide comprises SEQ ID NO:

18.

4. The isolated polypeptide of claim 1, wherein the polypeptide comprises the amino acid sequence selected from the group consisting of:

amino acid sequence from residue 22 to 150 of SEQ ID NO;

18, amino acid sequence from residue 23 to 150 of SEQ ID NO;

18, and amino acid sequence from residue 24 to 150 of SEQ ID NO;

18.

5. A composition comprising a polypeptide of claim 1 and a pharmaceutically acceptable carrier.

6. A polypeptide produced by culturing a recombinant host cell genetically engineered to contain an isolated polynucleotide encoding the polypeptide of claim 1, wherein the culturing is performed under conditions to promote expression of the polypeptide.