Method for producing biochemical analysis unit

• US 7,531,057 B2
• Filed: 01/31/2003
• Issued: 05/12/2009
• Est. Priority Date: 01/31/2002
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: Nakajima, Kenji, Kato, Akifumi
• Primary Examiner(s): SELLS, JAMES D
• Application Number:
  US10/354,994
• Publication Number:
  US 20030143523A1
• Time in Patent Office: 2,293 Days
• Field of Search: 156/250, 156/256, 156/261, 156/269, 156/555, 156/580, 156/582, 156/583.1
• US Class Current: 156/261
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00578 electrophoric
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B29C 35/0894 provided with masks or diaphragms
• CPC Class Codes: B29C 59/046 for layered or coated substantially flat surfaces
• CPC Class Codes: B32B 3/12 characterised by a layer of regularly- arranged cells, e.g. a honeycomb structure
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: Y10T 156/107 Punching and bonding pressure application by punch
• CPC Class Codes: Y10T 156/1084 of continuous or running length bonded web
• CPC Class Codes: Y10T 156/1741 Progressive continuous bonding press [e.g., roll couples]

Abstract

A continuous substrate and a continuous absorptive sheet are fed to a press station, and pressed continuously or intermittently. Part of the continuous absorptive sheet is charged in plural holes formed in the continuous substrate to form a biochemical analysis sheet. The biochemical analysis sheet is cut into predetermined tips to obtain a biochemical analysis unit.

Claims

1. A method for producing a biochemical analysis unit including a substrate having plural holes and an absorptive material provided in said holes, said method comprising steps of:

supplying a continuous substrate and a continuous absorptive sheet from supply rolls to a press station, said holes being formed in said continuous substrate, said continuous substrate being formed of materials for shielding a radioactive ray and a light, said continuous absorptive sheet being formed of said absorptive material;

pressing said continuous substrate and said continuous absorptive sheet at said press station, a part of said continuous absorptive sheet being charged in each of said holes of said substrate so as to form a biochemical analysis sheet; and

cutting said biochemical analysis sheet to obtain said biochemical analysis unit.

2. A method as described in claim 1, wherein said continuous substrate and said continuous absorptive sheet are fed intermittently, and the pressing by a pair of press plates is carried out while the feeding stops.

3. A method as described in claim 2, wherein one of surfaces of said continuous substrate is coated with an adhesive agent to adhere said continuous absorptive sheet to said continuous substrate, said adhesive agent contains at least one of styrene-butadiene rubber and acrylonitrile-butadiene rubber.

4. A method as described in claim 2, wherein a continuous adhesive agent sheet is positioned between said substrate and said absorptive sheet before pressing, said continuous adhesive sheet adheres said continuous absorptive sheet to said continuous substrate.

5. A method as described in claim 4, wherein said one press plate which contacts to said continuous substrate is heated, and a heating temperature of said press plates is higher than a temperature of glass transition temperature and lower than melting points of said adhesive agent, said continuous substrate and said continuous absorptive sheet.

6. A method as described in claim 5, wherein a surface roughness of said other press plate which contacts to said continuous absorptive sheet is more than 0.3 μ

m.

7. A method as described in claim 5, wherein a surface of said other press plate which contacts to said continuous absorptive sheet is coated with a hydrophobic resin or by plated with nickel or chromium containing said hydrophobic resin.

8. A method as described in claim 2, wherein said absorptive material in said holes is retracted from at least one of said surface of said substrate.

9. A method as described in claim 2, wherein said holes formed in said substrate are arranged at a predetermined pitch.

10. A method as described in claim 9, wherein said hole has a circular shape.

11. A method as described in claim 1, wherein the feeding of said continuous substrate and said continuous absorptive sheet are carried out continuously, and the pressing by press roller pair is carried out.

12. A method as described in claim 11, wherein one of surfaces of said continuous substrate is coated with an adhesive agent to adhere said continuous absorptive sheet to said continuous substrate, said adhesive agent contains at least one of styrene-butadiene rubber and acrylonitrile-butadiene rubber.

13. A method as described in claim 11, wherein a continuous adhesive agent sheet is positioned between said substrate and said absorptive sheet before pressing, said continuous adhesive sheet adheres said continuous absorptive sheet to said continuous substrate.

14. A method as described in claim 12, wherein said one press roller which contacts to said continuous substrate is heated, and a temperature of said press rollers is higher than a temperature of glass transition temperature and lower than melting points of said adhesive agent, said continuous substrate and said continuous absorptive sheet.

15. A method as described in claim 14, wherein a surface roughness of said other press roller which contacts to said continuous absorptive sheet is more than 0.3 μ

m.

16. A method as described in claim 14, wherein a surface of said other press roller which contacts to said continuous absorptive sheet is coated with a hydrophobic resin or by plated with nickel or chromium containing said hydrophobic resin.

17. A method as described in claim 11, wherein said absorptive material in said holes is retracted from at least one of said surface of said substrate.

18. A method as described in claim 11, wherein said holes formed in said substrate are arranged at a predetermined pitch.

19. A method as described in claim 18, wherein said hole has a circular shape.

20. A method for producing a biochemical analysis unit including a unit base having plural holes and an absorptive material provided in said holes, said method comprising steps of:

supplying a continuous substrate from first supply roll to a press station, said holes being formed in said continuous substrate, said continuous substrate being formed of materials for shielding a radioactive ray and a light;

supplying a continuous absorptive sheet to a press station from second supply roll which is different from said first supply roll, said continuous absorptive sheet being formed of said absorptive material;

pressing said continuous substrate and said continuous absorptive sheet at said press station, a part of said continuous absorptive sheet being charged in each of said holes of said substrate so as to form a biochemical analysis sheet; and

winding said biochemical analysis sheet to form a roll for use in making the biochemical analysis unit.

21. A method as described in claim 20, wherein a surface of said absorptive material is retracted from a surface of said substrate.