Interrogating multi-featured arrays

• US 7,531,303 B2
• Filed: 12/21/2001
• Issued: 05/12/2009
• Est. Priority Date: 10/29/1999
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Sillman, Debra A., Schleifer, Kyle J., White, Elecia C., Ladd, Charles S., Dorsel, Andreas N.
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US10/036,999
• Publication Number:
  US 20020058283A1
• Time in Patent Office: 2,699 Days
• Field of Search: 435/4, 435/6, 435/7.1, 435/174, 435/287.2, 422/68.1, 422/82.05
• US Class Current: 435/6.19
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 2201/1242 Validating, e.g. range invalidation, suspending operation

Abstract

A method, apparatus for executing the method, and computer program products for use in such an apparatus. The method includes scanning an interrogating light across multiple sites on an array package including an addressable array of multiple features of different moieties, which scanned sites include multiple array features. Signals from respective scanned sites emitted in response to the interrogating light are detected. The interrogating light power is altered for a first site on the array package during the array scan, based on location of the first site or on a determination that the emitted signal from the first site will be outside a predetermined value absent the altering (which allows for protecting a detector against expected overly bright sites), or is altered during the array scan based on the detected interrogating light power (which allows for compensating for light source drift during an array scan).

Claims

1. A method comprising:

(a) scanning an interrogating light across multiple sites on an array package including an addressable array of multiple features of different moieties, which scanned sites include multiple features of the array;

(b) detecting signals from respective scanned sites emitted in response to the interrogating light; and

(c) decreasing power of the interrogating light for a first site on the array package during a row scan of the interrogating light based on location of the first site, wherein the first site is outside an area occupied by the array.

2. A method comprising:

(a) scanning an interrogating light across multiple sites on an array package including an addressable array of multiple biopolymeric features of different moieties, which scanned sites include multiple features of the array;

(b) detecting signals from respective scanned sites emitted in response to the interrogating light; and

(c) altering power of the interrogating light for a first site on the away package during scanning;

wherein the first site is an array feature; and

wherein the interrogating light power is altered based on the signal emitted from the first site, when the interrogating light initially illuminates the first site.

3. A method comprising:

(a) prior to scanning an interrogating light across an away package, calibrating an interrogating light power versus a control signal characteristic from a light system which provides the interrogating light of a power which varies in response to the control signal characteristic;

(b) following step (a), scanning the interrogating light across multiple sites on an array package including an addressable away of multiple biopolymeric features of different moieties, which scanned sites include multiple features of the array;

(c) detecting signals from respective scanned sites emitted in response to the interrogating light; and

(d) altering the interrogating light power for a first site on the array package during the array scanning using the calibration of step (a), based on location of the first site or on a determination that the emitted signal from the first site will be outside a predetermined range absent the altering;

wherein the interrogating light power is altered during a row scan of the interrogating light.

4. A method according to claim 3 additionally comprising repeating steps (a) through (d) for each of multiple array packages.

5. A method according to claim 3 wherein the light system includes a light source and an optical attenuator through which light from the source passes to provide the interrogating light, and wherein the control signal comprises a signal for the optical attenuator which provides variable attenuation in response to the characteristic of the control signal.

6. A method according to claim 3 wherein the interrogating light power is reduced based on a determination that the emitted signal from the first site will exceed a predetermined value.

7. A method according to claim 6 wherein the determination is based on the emitted signal detected from the first site.

8. A method comprising:

(a) scanning an interrogating light across multiple sites on an array package including an addressable away of multiple biopolymeric features of different moieties, which scanned sites include multiple features of the array;

(b) detecting signals from respective scanned sites emitted in response to the interrogating light; and

(c) altering power of the interrogating light for a first site on the away package during array scanning based on location of the first site or on a determination that the emitted signal from the first site will be outside a predetermined intensity range absent the altering;

wherein the interrogating light power is altered during a row scan of the interrogating light.

9. A method according to claim 8 wherein the interrogating light power is reduced based on a determination that the emitted signal from the first site will exceed a predetermined value.

10. A method according to claim 8 wherein the interrogating light power is increased based on a determination that the emitted signal from the first site will be below a predetermined value.

11. A method according to claim 10 wherein the determination is based on the emitted signal detected from the first site.

12. A method according to claim 8 wherein the sites are multiple features of the array arranged in rows.

13. A method according to claim 8 wherein the interrogating light is scanned row by row across the array package.