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Use of representations of DNA for genetic analysis

  • US 7,531,307 B2
  • Filed: 03/30/2005
  • Issued: 05/12/2009
  • Est. Priority Date: 10/30/1997
  • Status: Expired due to Fees
First Claim
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1. A method of producing a compound representation of DNA, comprising:

  • (a) digesting a sample of DNA with a first restriction endonuclease to provide digested DNA fragments;

    (b) ligating an adaptor to said digested DNA fragments;

    (c) amplifying DNA fragments created in step b) using primers complementary to said adaptor to provide a first representation of said DNA;

    (d) digesting the first representation with a second restriction endonuclease to provide digested DNA fragments, wherein said second restriction endonuclease is different from said first restriction endonuclease; and

    (e) amplifying said DNA fragments of step (d) using primers which are the same as the primers used in step c) to provide a compound representation of said DNA.

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