Apparatus and method for transferring samples from a source to a target

• US 7,534,081 B2
• Filed: 05/24/2005
• Issued: 05/19/2009
• Est. Priority Date: 05/24/2005
• Status: Expired due to Fees

• Current Assignee: Festo Corp (Festo SE & Company KG)
• Original Assignee: Festo Corp (Festo SE & Company KG)
• Inventors: Porat, Amir, Leeman, Gerard William, Gombinsky, Moshe, Zobel, Hans-Jorg
• Primary Examiner(s): FOX, CHARLES A
• Application Number:
  US11/135,962
• Publication Number:
  US 20060269385A1
• Time in Patent Office: 1,456 Days
• Field of Search: 210/695, 414/222.01, 414/222.12
• US Class Current: 414/222.01
• CPC Class Codes: B01L 2200/0657 Pipetting powder
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/284 with associated cleaning means, e.g. retractable non-magnetic sleeve
• CPC Class Codes: B03C 1/286 disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 1/30 Combinations with other devices, not otherwise provided for
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical or biological applications
• CPC Class Codes: G01N 2035/0474 Details of actuating means for conveyors or pipettes
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom

Abstract

A control system for transferring a sample from a source vessel to a target vessel generally includes a vessel unit, a primary transfer unit, an x-drive, a y-drive, a z-drive and a control unit for controlling the drives. The vessel unit includes a support plate for supporting the source vessel and the target vessel thereon and the transfer unit includes at least one transfer device for transferring the sample from the source vessel to the target vessel. The x-, y- and z-drives reciprocally translate one of the support plate and the transfer device in a respective x-direction, y-direction and z-direction, wherein the x, y and z directions define a three axis Cartesian coordinate system.

Claims

1. A control system for transferring a sample from a source vessel to a target vessel, the control system comprising:

a vessel unit including a support plate for supporting the source vessel and the target vessel thereon;

a primary transfer unit including at least one transfer device for transferring the sample from the source vessel to the target vessel, said primary transfer unit including a primary magnet unit and said transfer device uses a magnetic force to attract the sample thereto, and wherein said transfer device further includes a hollow pin body terminating at a tip and a magnet slidably disposed within said hollow pin body, and said primary magnet unit further comprises a magnet actuator system for driving said magnet to move from a first position adjacent said tip, wherein said magnet applies said magnetic force through said tip, to a second position away from said tip, wherein said magnetic force is removed from said tip;

an x-axis drive adapted to reciprocally translate one of said support plate and said transfer device in an x-direction;

a y-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a y-direction;

a z-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a z-direction, said x, y and z directions defining a three axis Cartesian coordinate system; and

a control unit for controlling said x, y and z drives,

wherein said magnet actuator system comprises;

an actuator plate having at least one individually activated electromagnet disposed thereon;

an actuator plate drive for reciprocally translating said actuator plate; and

an actuator rod having a distal end connected to said magnet in said hollow pin body, said actuator rod including a ferromagnetic piston portion engageable with said electromagnet when said electromagnetic is activated for moving said magnet from said first position to said second position upon translation of said actuator plate.

2. A control system as defined in claim 1, wherein said primary magnet unit comprises an array of said pins and wherein said actuator system includes an array of electromagnets and actuator rods matching in number and arrangement with said pin array and wherein each pin of said pin array includes a magnet slidably disposed in a hollow pin body thereof and connected with a respective actuator rod.

3. A control system as defined in claim 1, wherein said actuator rod comprises a flexible cable.

4. A control system as defined in claim 1, wherein said actuator system further comprises a piston housing spaced from said actuator plate, said piston housing including a tension spring connected to said ferromagnetic piston portion of said actuator rod, for biasing said piston portion toward said piston housing.

5. A control system as defined in claim 1, wherein said magnet actuator system comprises:

an actuator rod having a proximal end and a distal end, said distal end being connected to said magnet in said hollow pin body; and

an individually activated rod drive connected to said proximal end of said actuator rod for moving said magnet from said first position to said second position.

6. A control system as defined in claim 1, further comprising a tip insertion unit for applying a disposable tip to said at least one transfer device of said primary transfer unit.

7. A control system as defined in claim 1, wherein said vessel drive unit is adapted for supporting at least one micro-well tray having at least one of the source vessel and the target vessel defined therein.

8. A control system for transferring a sample from a source vessel to a target vessel, the control system comprising:

a vessel unit including a support plate for supporting the source vessel and the target vessel thereon;

a primary transfer unit including at least one transfer device for transferring the sample from the source vessel to the target vessel, said primary transfer unit including a primary magnet unit and said transfer device uses a magnetic force to attract the sample thereto, and wherein said transfer device further includes a hollow pin body terminating at a tip and a magnet slidably disposed within said hollow pin body, and said primary magnet unit further comprises a magnet actuator system for driving said magnet to move from a first position adjacent said tip, wherein said magnet applies said magnetic force through said tip, to a second position away from said tip, wherein said magnetic force is removed from said tip;

an x-axis drive adapted to reciprocally translate one of said support plate and said transfer device in an x-direction;

a y-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a y-direction;

a z-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a z-direction, said x, y and z directions defining a three axis Cartesian coordinate system; and

a control unit for controlling said x, y and z drives,

wherein said magnet actuator system comprises;

an actuator rod having a proximal end and a distal end, said distal end being connected to said magnet in said hollow pin body, wherein said actuator rod comprises a flexible cable; and

an individually activated rod drive connected to said proximal end of said actuator rod for moving said magnet from said first position to said second position.

9. A control system for transferring a sample from a source vessel to a target vessel, the control system comprising:

a vessel unit including a support plate for supporting the source vessel and the target vessel thereon;

a primary transfer unit including at least one transfer device for transferring the sample from the source vessel to the target vessel;

an x-axis drive adapted to reciprocally translate one of said support plate and said transfer device in an x-direction;

a y-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a y-direction;

a z-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a z-direction, said x, y and z directions defining a three axis Cartesian coordinate system;

a control unit for controlling said x, y and z drives; and

a magnet unit including at least one magnet element supported on a magnet plate, said support plate of said vessel unit being disposed between said transfer device of said primary transfer unit and said magnet element of said magnet unit.

10. A control system as defined in claim 9, wherein said magnet unit further comprises a y-axis magnet plate drive for reciprocally translating said magnet plate in the y-direction and a z-axis magnet plate drive for reciprocally translating said magnet plate in the z-direction.

11. A control system as defined in claim 9, wherein said magnet element is adapted to be selectively activated and de-activated for alternately applying and removing a magnetic field at a bottom of at least one of the source vessel and the target vessel.

12. A control system as defined in claim 11, wherein said magnet unit further comprises:

an array of magnet elements; and

a magnet actuator system for selectively activating and de-activating at least one magnet element of said array.

13. A control system as defined in claim 11, wherein said magnet element comprises a magnet slidably disposed in a bore of said magnet plate, and wherein said magnet unit further comprises a magnet actuator system for driving said magnet to move from a first position adjacent said vessel support plate for applying a magnetic force to at least one of the source vessel and the target vessel, to a second position away from said vessel support plate for removing said magnetic force from said at least one of the source vessel and the target vessel.

14. A control system as defined in claim 13, wherein said magnet actuator system comprises:

an actuator plate having at least one individually activated electromagnet disposed thereon;

an actuator plate drive for reciprocally translating said actuator plate; and

a magnet rod having a distal end connected to said magnet in said magnet plate, said magnet rod including a ferromagnetic piston portion engageable with said electromagnet when said electromagnet is activated for moving said magnet from said first position to said second position upon translation of said actuator plate.

15. A control system as defined in claim 14, wherein said actuator system includes an array of electromagnets and magnet rods matching in number and arrangement with said magnet element array, and wherein each magnet element of said array includes a magnet slidably disposed in a bore of said magnet plate and connected with a respective magnet rod.

16. A control system as defined in claim 14, wherein said magnet rod comprises a flexible cable.

17. A control system as defined in claim 14, wherein said magnet actuator system further comprises a piston housing spaced from said actuator plate, said piston housing including a tension spring connected to said ferromagnetic piston portion of said magnet rod, for biasing said piston portion toward said piston housing.

18. A control system as defined in claim 13, wherein said magnet actuator system comprises:

a magnet rod having a proximal end and a distal end, said distal end being connected to said magnet in said bore of said magnet plate; and

an individually activated magnet rod drive connected to said proximal end of said magnet rod for moving said magnet from said first position to said second position.

19. A control system for transferring a sample from a source vessel to a target vessel, the control system comprising:

a vessel unit including a support plate for supporting the source vessel and the target vessel thereon;

a primary transfer unit including at least one transfer device for transferring the sample from the source vessel to the target vessel;

an x-axis drive adapted to reciprocally translate one of said support plate and said transfer device in an x-direction;

a y-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a y-direction;

a z-axis drive adapted to reciprocally translate one of said support plate and said transfer device in a z-direction, said x, y and z directions defining a three axis Cartesian coordinate system;

a control unit for controlling said x, y and z drives; and

a tip insertion unit for applying a disposable tip to said at least one transfer device of said primary transfer unit, wherein said tip insertion unit comprises;

a block having a bore formed therein, said bore having a proximal end and a distal end, said proximal end being sized to receive a disposable tip for application to said transfer device; and

a pressure source connected to said distal end of said bore for applying a pressure in said bore for forcing the disposable tip out of said bore.

20. A control system as defined in claim 19, wherein said block further includes a piston slidably received within said bore, said piston being driven by said pressure applied by said pressure source to force the disposable tip out of said bore.

21. A control system as defined in claim 19, further comprising a tip removal unit for removing a disposable tip from said at least one transfer device of said primary transfer unit.

22. A control system as defined in claim 21, wherein said tip removal unit comprises a fork defined by at least one channel having a width corresponding to a diameter of said transfer device, said channel being adapted to engage a disposable tip of said transfer device when said transfer device is brought into said channel.

23. A system for transferring a sample from a source vessel to a target vessel, the system comprising:

a transfer device having a hollow body terminating at a tip and an actuating element movably disposed in said hollow body between a first position adjacent said tip and a second position away from said tip, said movement of said actuating element causing a sample in proximity to said transfer device to be alternately collected and released from said transfer device;

an actuator plate having at least one individually activated electromagnet disposed thereon;

an actuator plate drive for reciprocally translating said actuator plate; and

an actuator rod having a distal end connected to said actuating element in said hollow body of said transfer device, said actuator rod including a ferromagnetic piston portion engageable with said electromagnet when said electromagnetic is activated for moving said actuating element from said first position to said second position upon translation of said actuator plate.

24. A system as defined in claim 23, wherein said actuating element of said transfer device is a magnet slidably disposed within said hollow body between said first position and said second position, wherein a magnetic force is applied at said tip when said magnet is in said first position, and wherein said magnetic force is removed from said tip when said magnet is in said second position for alternately collecting and releasing samples from said transfer device.

25. A system as defined in claim 23, wherein said actuator rod comprises a flexible cable.

26. A system as defined in claim 23, further comprising a piston housing spaced from said actuator plate, said piston housing including a tension spring connected to said ferromagnetic piston portion of said actuator rod, for biasing said piston portion toward said piston housing.