Analyte measurement
First Claim
1. A method for determining the concentration of an analyte in a sample which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, said reaction going to completion or substantially to completion before a potential is applied, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.
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Abstract
A method for determining the concentration of an analyte in a sample is disclosed which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.
16 Citations
25 Claims
- 1. A method for determining the concentration of an analyte in a sample which comprises contacting the sample with a micro electrode which comprises an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, said reaction going to completion or substantially to completion before a potential is applied, then applying a potential across the electrode and measuring the resulting concentration of the converted mediator electrochemically.
- 14. A method of determining the concentration of an analyte in a sample which comprises contacting the sample with a micro electrode which contains an enzyme capable of reacting with said analyte and a redox mediator which is capable of being converted by being oxidised or reduced by said enzyme once the latter has reacted with the analyte, allowing the analyte to react with the enzyme, then applying a potential across the electrode, measuring the resulting current, reversing the potential and measuring the current again, expressing the two currents as a ratio or a percentage and determining the concentration of the analyte directly therefrom.
Specification