Polymer grafting by polysaccharide synthases
First Claim
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1. A method for elongating a functional acceptor, comprising the steps of:
- providing a functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of GlcUA, GlcNAc, GalNAc, and hexosamine, and wherein the functional acceptor is attached to a substrate selected from the group consisting of silica, silicon, glass, polymers, organic compounds, metals and combinations thereof;
providing a recombinant, soluble hyaluronic acid synthase having an empty acceptor site and being capable of elongating the functional acceptor, wherein the recombinant, soluble hyaluronic acid synthase has the amino acid sequence as set forth in SEQ ID NO;
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providing UDP-GlcUA and UDP-GlcNAc sugars such that the recombinant, soluble hyaluronic acid synthase elongates the functional acceptor.
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Abstract
The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate synthase from Pasteurella multocida.
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Citations
8 Claims
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1. A method for elongating a functional acceptor, comprising the steps of:
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providing a functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of GlcUA, GlcNAc, GalNAc, and hexosamine, and wherein the functional acceptor is attached to a substrate selected from the group consisting of silica, silicon, glass, polymers, organic compounds, metals and combinations thereof; providing a recombinant, soluble hyaluronic acid synthase having an empty acceptor site and being capable of elongating the functional acceptor, wherein the recombinant, soluble hyaluronic acid synthase has the amino acid sequence as set forth in SEQ ID NO;
1; andproviding UDP-GlcUA and UDP-GlcNAc sugars such that the recombinant, soluble hyaluronic acid synthase elongates the functional acceptor. - View Dependent Claims (2, 3, 4)
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5. A method for elongating a functional acceptor, comprising the steps of:
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providing a functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of GlcUA, GlcNAc, GalNAc, and hexosamine, and wherein the functional acceptor is attached to a substrate selected from the group consisting of silica, silicon, glass, polymers, organic compounds, metals and combinations thereof; providing a recombinant, soluble hyaluronic acid synthase having an empty acceptor site and being capable of elongating the functional acceptor, wherein the recombinant, soluble hyaluronic acid synthase is encoded by a nucleotide sequence which is at least 90% identical to SEQ ID NO;
2; andproviding UDP-GlcUA and UDP-GlcNAc sugars such that the recombinant, soluble hyaluronic acid synthase elongates the functional acceptor. - View Dependent Claims (6, 7, 8)
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Specification