Method of producing cardiomyocytes from mesenchymal stem cells

• US 7,534,607 B1
• Filed: 12/27/2005
• Issued: 05/19/2009
• Est. Priority Date: 12/27/2005
• Status: Expired due to Fees

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: Lin, Su-Yo, Chen, Wann-Hsin
• Primary Examiner(s): Hama; Joanne
• Application Number:
  US11/319,113
• Time in Patent Office: 1,239 Days
• Field of Search: None
• US Class Current: 435/377
• CPC Class Codes: C12N 2500/38 Vitamins
• CPC Class Codes: C12N 5/0657 Cardiomyocytes; Heart cells

Abstract

Methods are provided for producing cardiomyocytes suitable for various medical applications. In one embodiment, a method of producing cardiomyocytes is provided, which comprises culturing mesenchymal stem cells in a medium containing ascorbic acid. In another embodiment, such method further comprises the step of selecting fast-growing mesenchymal stem cell clones before culturing the selected clones in the culturing medium that contains ascorbic acid.

Claims

1. A method of producing cardiomyocytes comprising the steps of selecting mammalian mesenchymal stem cell clones that have a doubling time of less than 25 hours and administering ascorbic acid as a sole differentiating agent to induce differentiation of the mesenchymal stem cells into cardiomyocytes.

2. The method of claim 1, wherein the amount of ascorbic acid is between 100 μ

M to 10 mM.

3. The method of claim 2, wherein the amount of ascorbic acid is about 1 mM.