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Methods of producing pancreatic hormones

  • US 7,534,608 B2
  • Filed: 07/02/2008
  • Issued: 05/19/2009
  • Est. Priority Date: 07/26/2006
  • Status: Active Grant
First Claim
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1. A method for producing insulin, said method comprising the steps of:

  • (a) contacting human embryonic stem (hES) cells in vitro with a first medium comprising activin A, thereby activating a TGF-β

    receptor family member;

    (b) culturing in vitro the hES-derived cells of step (a) in a second medium lacking activin A, thereby generating PDX-1 positive pancreatic endoderm cells;

    (c) transplanting the PDX-1 positive pancreatic endoderm cells of step (b) into a mammalian subject; and

    (d) maturing the PDX-1 positive pancreatic endoderm cells of step (c) in vivo, thereby obtaining insulin secreting cells, wherein the insulin secreting cells secrete insulin in response to glucose stimulation.

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