Method for preparing and implanting a cartilage construct to treat cartilage lesions

• US 7,537,780 B2
• Filed: 07/22/2003
• Issued: 05/26/2009
• Est. Priority Date: 03/22/2002
• Status: Expired due to Term

• Current Assignee: Histogenics Corporation (Ocugen, Inc.), Takagi Industrial Company Limited
• Original Assignee: Histogenics Corporation (Ocugen, Inc.)
• Inventors: Kusanagi, Akihiko, Tarrant, Laurence J. B., Tokuno, Toshimasa, Smith, Robert Lane, Mizuno, Shuichi
• Primary Examiner(s): Naff; David M
• Application Number:
  US10/626,459
• Publication Number:
  US 20040191900A1
• Time in Patent Office: 2,135 Days
• Field of Search: None
• US Class Current: 424/423
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3817 Cartilage-forming cells, e.g. pre-chondrocytes
• CPC Class Codes: A61L 27/3843 Connective tissue
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: C12N 5/0655 Chondrocytes; Cartilage

Abstract

Neo-cartilage constructs for growth and surface coating for the de novo formation of a superficial cartilage layer. A method for generation of the neo-cartilage and the neo-cartilage construct. A method for repair and restoration of the injured, damaged, diseased or aged cartilage into its full functionality and for treatment of injured or diseased cartilage by implanting the neo-cartilage construct between two layers of biologically acceptable sealants.

Claims

1. A method for treatment of cartilage lesions comprising steps:

a) isolating autologous or heterologous chondrocytes or prviding stem cells that can be differentiated into chondrocytes;

b) expanding said isolated chondrocytes or stem cells in a growth medium;

c) suspending said expanded chondrocytes or stem cells in a collagen-containing solution;

d) providing a biodegradable support matrix containing plurality of pores;

e) incorporating the suspension obtained in step c) into said support matrix thereby producing a seeded support matrix;

f) preparing an implantable construct for implantation into said cartilage lesion by activating said chondrocytes or stem cells by subjecting said seeded support matrix to conditions promoting activation and propagation of said chondrocytes or stem cells within said support matrix, wherein said conditions for activation and propagation of chondrocytes or stem cells comprise

perfusing said seeded support matrix with a culture medium at a flow rate from about 1 μ

L/min to about 500 μ

L/min and

during said perfusing, applying to said seeded support matrix a cyclic hydrostatic pressure from about 0.01 MPa to about 10 MPa above atmospheric pressure at about 0.01 to about 1 Hz for from about 1 to about 8 hours followed by applying a static atmospheric pressure for from 16 to about 23 hours;

g) implanting said construct into said cartilage lesion; and

h) depositing a biodegradable polyethylene glycol cross-linked with methylated collagen over said construct,

wherein said implanting of said construct into said cartilage lesion and said deposition of said polyethylene glycol cross-linked with methylated collagen over said implanted construct results, within three months, in integration of said construct into a native cartilage of the lesion and in formation of a superficial cartilage layer that overgrows said polyethylene glycol cross-linked with methylated collagen.

2. The method of claim 1 further comprising step i) wherein a tissue sealant is deposited into said cartilage lesion before said construct is implanted therein,

wherein said sealant is selected from the group consisting of gelatin, a copolymer of polyethylene glycol and poly-lactide or poly-glycolide, periodate-oxidized gelatin, 4-armed pentaerythritol thiol and a polyethylene glycol diacrylate, 4-armed tetra-succinimidyl ester or tetra-thiol derivatized PEG, photo-polymerizable polyethylene glycol-co-poly(α

-hydroxy acid) diacrylate macromer, 4-armed polyethylene glycol derivatized with succinimidyl ester and thiol further cross-linked with methylated collagen, derivatized polyethylene glycol (PEG), polyethylene glycol (PEG) cross-linked with alkylated collagen, tetra-hydrosuccinimidyl or tetra-thiol derivatized PEG, PEG cross-linked with methylated collagen, and a combination thereof.

3. The method of claim 1 wherein said support matrix is in the form of a sponge or honeycomb prepared from a compound selected from the group consisting of a Type I collagen, Type II collagen, Type IV collagen, gelatin, agarose, collagen containing proteoglycan, collagen containing glycosaminoglycan and collagen containing glycoprotein.

4. The method of claim 3 wherein said support matrix is a prepared from Type I collagen.

5. The method of claim 1 wherein said hydrostatic cyclic pressure is applied from about 0.05 MPa to about 3 MPa at 0.1 to about 0.5 Hz.

6. The method of claim 1 wherein said perfusion flow rate is from about 5 μ

L/min to about 50 μ

L/min.

7. The method of claim 6 wherein said perfusion flow rate is about 5μ

L/min.

8. The method of claim 1 wherein cell activation is performed under oxygen concentration from about 1% to about 20%.

9. The method of claim 8 wherein cell activation is performed under oxygen concentration from about 2% to about 5%.

10. The method of claim 1 wherein said formation of superficial cartilage layer constitutes forming a substitute synovial membrane.