Parthenolide free bioactive ingredients from feverfew (Tanacetum parthenium) and processes for their production

• US 7,537,791 B2
• Filed: 02/21/2007
• Issued: 05/26/2009
• Est. Priority Date: 02/21/2006
• Status: Active Grant

• Current Assignee: Isp Investments LLC (Ashland Global Holdings, Inc.)
• Original Assignee: Integrated Botanical Technologies, LLC
• Inventors: Koganov, Michael
• Primary Examiner(s): Tate; Christopher R.
• Assistant Examiner(s): Davis; Deborah A.
• Application Number:
  US11/677,435
• Publication Number:
  US 20070196523A1
• Time in Patent Office: 825 Days
• Field of Search: None
• US Class Current: 424/764
• CPC Class Codes: A61K 36/28 Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/16 Emollients or protectives, e.g. against radiation
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 25/06 Antimigraine agents
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants

Abstract

The present invention relates to bioactive ingredients that include isolated bioactive fractions derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant. The bioactive fractions are either free of or substantially free of α-unsaturated γ-lactones (e.g., parthenolide). Further, the bioactive fractions have anti-inflammatory and antioxidant activity. The present invention also relates to a method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant and that is at least substantially free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a method for preparing a stabilized cell juice serum fraction and a stabilized concentrated cell juice serum fraction that are free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a bioactive composition that includes a mixture of one or more of the isolated bioactive fractions of the present invention.

Claims

1. A method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant and that is substantially free of α

-unsaturated γ

-lactones, said method comprising;

providing fresh biomass of a feverfew (Tanacetum parthenium) plant;

processing the fresh biomass under conditions effective to yield a cell juice supernatant that is at least substantially free of α

-unsaturated γ

-lactones and a membrane fraction containing α

-unsaturated γ

-lactones, said processing the fresh biomass comprising;

(i) separating the fresh biomass into a cell juice component and a fiber enriched material; and

(ii) subjecting the cell juice component to a combined pH adjustment and microwave treatment step to yield the cell juice supernatant that is at least substantially free of α

-unsaturated γ

-lactones, wherein the pH adjustment comprises decreasing the pH to a range of 3 to 4;

treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; and

isolating the cytoplasm fraction precipitate, wherein said cytoplasm fraction precipitate is a bioactive fraction that is substantially free of α

-unsaturated γ

-lactones, wherein said α

-unsaturated γ

-lactones comprise parthenolide.

2. The method according to claim 1, wherein the step of processing the fresh biomass further comprises:

clarifying the cell juice component to yield a clarified cell juice component prior to subjecting the cell juice component to the combined pH adjustment and microwave treatment step.

3. The method according to claim 1 further comprising:

clarifying the first cell juice serum supernatant under conditions effective to yield a second cell juice serum supernatant;

mixing a stabilizing agent with the second cell juice serum supernatant under conditions effective to yield a stabilized cell juice serum fraction,

wherein said stabilized cell juice serum fraction is a bioactive fraction that is substantially free of α

-unsaturated γ

-lactones, said α

-unsaturated γ

-lactones comprising parthenolide.

4. The method according to claim 3 further comprising:

concentrating the stabilized cell juice serum fraction under conditions effective to yield a concentrated cell juice serum supernatant.

5. The method according to claim 4 further comprising:

mixing a stabilizing agent with the concentrated cell juice serum supernatant under conditions effective to yield a stabilized concentrated cell juice serum fraction,

wherein said stabilized concentrated cell juice serum fraction is a bioactive fraction that is free of α

-unsaturated γ

-lactones, said α

-unsaturated γ

-lactones comprising parthenolide.