Method for the production of polyunsaturated fatty acids
First Claim
1. An isolated nucleic acid coding for a polypeptide having acyl-CoA:
- lysophospholipid-acyltransferase activity, wherein the isolated nucleic acid comprises a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO;
1, or encodes a polypeptide having at least 95% identity to the polypeptide sequence of SEQ ID NO;
2, wherein the acyl-CoA;
lysophospholipid acyltransferase encoded by said nucleic acid specifically uses C16, C18-, C20- or C22-fatty acids having at least one double bond as substrate.
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Abstract
The present invention relates to a process for producing polyunsaturated fatty acids in an organism by introducing nucleic acids into the organism which code for polypeptides having acyl-CoA:lysophospholipid a cyltransferase activity. Advantageously, these nucleic acid sequences may, if appropriate together with further nucleic acid sequences coding for biosynthesis polypeptides of the fatty acid or lipid metabolism, be expressed in the transgenic organism. The invention furthermore relates to the nucleic acid sequences, to nucleic acid constructs comprising the nucleic acid sequences of the invention, to vectors comprising the nucleic acid sequences and/or the nucleic acid constructs and to transgenic organisms comprising the abovementioned nucleic acid sequences, nucleic acid constructs and/or vectors. A further part of the invention relates to oils, lipids and/or fatty acids produced by the process of the invention and to their use.
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Citations
17 Claims
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1. An isolated nucleic acid coding for a polypeptide having acyl-CoA:
- lysophospholipid-acyltransferase activity, wherein the isolated nucleic acid comprises a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO;
1, or encodes a polypeptide having at least 95% identity to the polypeptide sequence of SEQ ID NO;
2, wherein the acyl-CoA;
lysophospholipid acyltransferase encoded by said nucleic acid specifically uses C16, C18-, C20- or C22-fatty acids having at least one double bond as substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- lysophospholipid-acyltransferase activity, wherein the isolated nucleic acid comprises a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO;
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9. A process for producing polyunsaturated fatty acids in an organism, wherein said process comprises:
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a) introducing into an organism at least one nucleic acid coding for a polypeptide having acyl-CoA;
lysophospholipid-acyltransferase activity, andb) culturing and harvesting said organism, wherein the nucleic acid comprises a nucleotide sequence selected from the group consisting of; i) the nucleotide sequence of SEQ ID NO;
1,ii) a nucleotide sequence having at least 95% identity to the nucleotide sequence of SEQ ID NO;
1,iii) a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO;
2, andiv) a nucleotide sequence encoding a polypeptide having at least 95% identity to the polypeptide sequence of SEQ ID NO;
2. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17)
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Specification