Method of testing multiple fluid samples with multiple biopolymer arrays

• US 7,537,936 B2
• Filed: 05/23/2005
• Issued: 05/26/2009
• Est. Priority Date: 05/31/2002
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Schleifer, Arthur, Schembri, Carol T., Dahm, SueAnn C., Amorese, Douglas A.
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US11/136,227
• Publication Number:
  US 20050214854A1
• Time in Patent Office: 1,464 Days
• Field of Search: 436/180, 422/50, 422/68.1, 422/99
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A method of testing multiple fluid samples with multiple biopolymer arrays. A cover is assembled to a contiguous substrate carrying on a first side, multiple arrays each with multiple regions of biopolymers linked to the substrate, such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own port. Multiple fluid samples are introduced into respective chambers through a port of each such that the fluid samples contact respective arrays. A binding pattern of the arrays is observed. An apparatus and kit useful in such methods, are also provided.

Claims

1. A method of testing multiple fluid samples using a contiguous substrate carrying multiple arrays each with multiple discrete regions of biopolymers linked to the substrate, comprising:

exposing at least one of the arrays to a test sample under a first set of conditions;

and exposing at least another one of the arrays to a reference sample under the same set of conditions, wherein said at least another one of the arrays is not exposed to any test sample; and

assembling a cover to the first side of the substrate such that the cover and the substrate together form a plurality of chambers each containing a biopolymer array and each being accessible through its own first and second ports positioned on the same side of said substrate.

2. A method according to claim 1 additionally comprising observing a binding pattern of the arrays and, when an observed characteristic of the binding pattern of an array exposed to the reference sample is outside a predetermined limit, either rejecting the binding pattern result for the test sample or modifying observed binding pattern results for the test sample based on a difference between an expected and observed characteristic of an array exposed to the reference sample.

3. A method according to claim 1 wherein:

the array exposed to the test sample and the array exposed to the reference sample both include at least one reference feature, and wherein both are exposed to at least one reference sequence;

the method additionally comprising observing a binding pattern of the arrays;

and wherein when the binding pattern of the at least one reference feature in both arrays lacks a predetermined degree of correlation, either rejecting the binding pattern result for the test sample or modifying an observed binding pattern results for the test sample based on a difference between an expected and observed correlation.

4. A method according to claim 3 wherein the at least one reference feature included in the array exposed to the test sample and the at least one reference feature included in the array exposed to the reference sample are identical.

5. A method according to claim 4 wherein the at least one reference sequence exposed to the array exposed to the test sample and the at least one reference sequence exposed to the array exposed to the reference sample are identical.

6. A method according to claim 5 wherein the predetermined degree of correlation is the predetermined degree of similarity to the observed binding pattern of the at least one reference feature in both arrays.

7. A method according to claim 1 wherein the at least one array exposed to the test sample is also exposed to a reference sample.

8. A method according to claim 7 wherein the reference sample exposed to the at least one array exposed to the test sample and the reference sample exposed to the at least another one of the arrays are identical.

9. A method according to claim 1 wherein multiple test samples are exposed to multiple respective arrays on the contiguous substrate.

10. A method according to claim 9 wherein the multiple test samples are different from each other.

11. A method according to claim 1, wherein aid biopolymers are nucleic acids.

12. A method according to claim 1, further comprising introducing fluid into at least one of said chambers through one of the first and second ports of said at least one chamber while venting through the other of the first and second ports of said at least one chamber.