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Cleavage of nucleic acids

  • US 7,541,145 B2
  • Filed: 07/15/2005
  • Issued: 06/02/2009
  • Est. Priority Date: 01/24/1996
  • Status: Expired due to Fees
First Claim
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1. A method for detecting a target nucleic acid comprising:

  • exposing a composition comprising a purified thermostable FEN-1 endonuclease from an archaebacterial species selected from the group consisting of Pyrococcus furiosus and Methanococcus jannaschii and a purified nucleic acid molecule, wherein said purified nucleic acid molecule is capable of hybridizing to a target nucleic acid to form a FEN-1 endonuclease cleavage structure, to a sample suspected of containing said target nucleic acid;

    forming a FEN-1 endonuclease cleavage structure; and

    cleaving said FEN-1 endonuclease cleavage structure with said purified thermostable FEN-1 endonuclease, such that said target nucleic acid is detected.

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