Apparatus for the pH determination of blood and method therefor
First Claim
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1. A spectroscopic analysis apparatus for non-invasive in vivo analysis of blood comprising:
- an excitation system for emitting an excitation beam to excite a target region containing a blood vessel, said excitation system configured to emit an excitation beam having a predetermined polarization orientation;
a detection system for detecting scattered radiation from blood in the blood vessel in the tar et re ion generated b the excitation beam to obtain a Raman signal;
a signal processing system for extracting at least one predetermined pH sensitive signal portion from said Raman signal and for determining a depolarization ratio from said at least one pH sensitive signal portion of said Raman signal and for determining the pH value of the blood in the target region from said depolarization ratio by use of a relationship between pH value and depolarization ratio of vibrations of one or more molecules at excitation wavelengths included in said at least one pH sensitive signal portions;
a monitoring system for imaging the target region; and
a focusing system for focusing the excitation system, the detection system and the monitoring system on the target region.
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Abstract
The present invention relates to a spectroscopic apparatus for non-invasive in vivo analysis of blood and to a corresponding analysis method, in particular for direct determination of the pH value of the blood. From a Raman signal detected from an excited target region containing blood at least one predetermined pH sensitive signal portion is determined, from which the pH value of the blood in the target region by use of a relationship between pH value and one or more band parameters of pH sensitive vibrations of at least one molecule or part of a molecule present in blood, in particular heme vibrations of hemoglobin, is determined.
10 Citations
18 Claims
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1. A spectroscopic analysis apparatus for non-invasive in vivo analysis of blood comprising:
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an excitation system for emitting an excitation beam to excite a target region containing a blood vessel, said excitation system configured to emit an excitation beam having a predetermined polarization orientation; a detection system for detecting scattered radiation from blood in the blood vessel in the tar et re ion generated b the excitation beam to obtain a Raman signal; a signal processing system for extracting at least one predetermined pH sensitive signal portion from said Raman signal and for determining a depolarization ratio from said at least one pH sensitive signal portion of said Raman signal and for determining the pH value of the blood in the target region from said depolarization ratio by use of a relationship between pH value and depolarization ratio of vibrations of one or more molecules at excitation wavelengths included in said at least one pH sensitive signal portions; a monitoring system for imaging the target region; and a focusing system for focusing the excitation system, the detection system and the monitoring system on the target region. - View Dependent Claims (2, 3, 4, 5)
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6. A spectroscopic analysis apparatus for non-invasive in vivo analysis of blood comprising:
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an excitation system for emitting an excitation beam to excite a target region containing blood; a detection system for detecting scattered radiation from the target region generated by the excitation beam to obtain a Raman signal; a signal processing system for extracting at least one predetermined pH sensitive signal portion from said Raman signal and for determining the pH value of the blood in the target region from said at least one pH sensitive signal portion by use of a relationship between pH value and one or more band parameters of pH sensitive vibration of at least one molecule or part of a molecule present in blood; a monitoring system for imaging the target region; a focusing system for focusing the excitation system, the detection system and the monitoring system on the target region on a blood vessel; and an auto focus system which continually adjusts a confocal center of the excitation beam. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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15. A spectroscopic analysis apparatus including:
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an optical detection system including; an excitation s stem configured to emit an excitation beam to excite blood in a blood vessel below a surface of a subject, a detection system configured to detect scattered radiation from the blood in the blood vessel to generate a Raman signal, a signal processing system configured to determine a pH value of the blood in the blood vessel from the Raman signal; an optical monitoring system including; an optical light source configured to emit a monitoring beam that penetrates to the blood vessel, a camera configured to receive the monitoring beam from the blood vessel and form an image of the blood vessel; a microscope object which focuses the excitation and monitoring beams into the subject, the microscope object hem controllable in accordance with the image to focus at least the excitation beam on the blood vessel; and an auto focus mechanism connected with the camera and the microscope object to adjust the focus of the microscope object to maintain a confocal detection center of the excitation beam focused on the blood vessel when the subject moves. - View Dependent Claims (16, 17, 18)
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Specification