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System and method for heating, cooling and heat cycling on microfluidic device

  • US 7,544,506 B2
  • Filed: 06/07/2004
  • Issued: 06/09/2009
  • Est. Priority Date: 06/06/2003
  • Status: Active Grant
First Claim
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1. A thermocycling apparatus for driving one complete polymerase chain reaction temperature cycle in less than or about 15 seconds, comprising:

  • a) a plastic card body comprising;

    i) a first plate with a first surface and a second surface,ii) a first amplification chamber formed in said first surface,iii) a microfluidic assay fluid circuit fluidly connected to said first amplification chamber, andiv) a plastic heat exchange film covering and sealing over said first amplification chamber, said plastic heat exchange film with interior surface facing said first surface of said first plate and with oppositely facing exterior surface; and

    b) a first heat transfer element in thermal contact with said exterior surface of said plastic heat exchange film over said first amplification chamber,wherein said plastic heat exchange film is sealed to said first surface with a double-sided ACA adhesive layer.

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