Stem cells of the islets of Langerhans and their use in treating diabetes mellitus
First Claim
1. A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
- (a) removing a pancreatic islet from a donor;
(b) culturing cells from the pancreatic islet under conditions wherein said cultured cells comprise nestin-positive cells which have migrated from said islet; and
(c) selecting said migrated nestin-positive cells from the culture, wherein said migrated cells from step (b) form a monolayer.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
-
Citations
2 Claims
-
1. A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
-
(a) removing a pancreatic islet from a donor; (b) culturing cells from the pancreatic islet under conditions wherein said cultured cells comprise nestin-positive cells which have migrated from said islet; and (c) selecting said migrated nestin-positive cells from the culture, wherein said migrated cells from step (b) form a monolayer.
-
-
2. A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
-
(a) removing a pancreatic islet from a donor; (b) culturing cells from the pancreatic islet in a first vessel coated with concanavalin A to separate concanavalin A adherent and non-adherent cells; (c) transferring said non-adherent cells from step (b) to a second vessel not coated with concanavalin A; (d) culturing cells from step (c) in said second vessel to produce a cell culture wherein said nestin-positive cells adhere to said second vessel and wherein said nestin-positive cells form a monolayer; and (e) selecting said nestin-positive cells that have formed a monolayer from step (d) to produce an isolated stem cell.
-
Specification