Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
First Claim
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1. A conjugate, comprising a nucleotide attached to a fluorescent marker through a photocleavable linker, said marker comprising BODIPY.
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Abstract
Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.
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10 Claims
- 1. A conjugate, comprising a nucleotide attached to a fluorescent marker through a photocleavable linker, said marker comprising BODIPY.
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