Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
First Claim
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1. A pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase (s-GDH) mutant, comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 24 and wherein said mutant comprises an alanine or a serine substitution for threonine at position 348 of SEQ ID NO;
24 and wherein the mutant has s-GDH activity.
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Abstract
The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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Citations
11 Claims
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1. A pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase (s-GDH) mutant, comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 24 and wherein said mutant comprises an alanine or a serine substitution for threonine at position 348 of SEQ ID NO;
24 and wherein the mutant has s-GDH activity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 11)
- 24 and wherein said mutant comprises an alanine or a serine substitution for threonine at position 348 of SEQ ID NO;
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9. A PQQ-dependent s-GDH mutant, said mutant comprising an amino acid sequence at least 90% identical to SEQ ID NO:
- 24 wherein said mutant comprises a serine substitution for threonine at position 348 of SEQ ID NO;
24 and wherein the mutant has s-GDH activity.
- 24 wherein said mutant comprises a serine substitution for threonine at position 348 of SEQ ID NO;
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10. A PQQ-dependent s-GDH mutant, said mutant comprising an amino acid sequence at least 90% identical to SEQ ID NO:
- 24 wherein said mutant comprises an alanine substitution for threonine at position 348 of SEQ ID NO;
24 and wherein the mutant has s-GDH activity.
- 24 wherein said mutant comprises an alanine substitution for threonine at position 348 of SEQ ID NO;
Specification