Method and apparatus for compact spectrometer for multipoint sampling of an object

• US 7,548,310 B2
• Filed: 03/03/2006
• Issued: 06/16/2009
• Est. Priority Date: 07/18/2003
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Vanni, G. Steven, Demuth, Joseph E., Wang, Chenhui, Nelson, Matthew P., Treado, Patrick J., Schweitzer, Robert C., Wolfe, Julianne, Gardner, Charles W. Jr., Neiss, Jason H., Maier, John S.
• Primary Examiner(s): Geisel; Kara E
• Application Number:
  US11/366,660
• Publication Number:
  US 20060268266A1
• Time in Patent Office: 1,201 Days
• Field of Search: 356/301
• US Class Current: 356/301
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 2201/0221 Portable; cableless; compact; hand-held

Abstract

The disclosure relates to methods and apparatus for assessing occurrence of one or more hazardous agents in a sample by performing multipoint spectral analysis of the sample using a portable or hand-held device. Methods of employing Raman spectroscopy and other spectrophotometric methods are disclosed. Devices and systems suitable for performing such multipoint methods are also disclosed.

Claims

1. A method of assessing occurrence of an agent in a sample, comprising the steps of:

(a) illuminating the sample with photons emanating from a portable device to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship; and

(b) simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device wherein said Raman-scattered photons are characteristic of the presence or absence of the agent in the sample.

2. The method of claim 1 wherein the number of points in the first set of multiple points is three.

3. The method of claim 1 wherein the number of points in the first set of multiple points is greater than or equal to four and less than or equal to ten.

4. The method of claim 1 wherein the number of points in the first set of multiple points is greater than or equal to ten and less than or equal to fifty.

5. The method of claim 1 wherein the number of points in the first set of multiple points is greater than fifty.

6. The method of claim 1 further comprising the steps of:

(c) illuminating the sample with photons emanating from the portable device to thereby produce Raman-scattered photons from a second set of multiple points in the sample wherein said second set of multiple points corresponds to a point in said first set of multiple points from which said Raman-scattered photons are characteristic of the presence of the agent; and

(d) simultaneously analyzing the Raman-scattered photons from said second set of multiple points in the sample using the portable device wherein said Raman-scattered photons from said second set of multiple points are characteristic of the presence or absence of the agent in the sample.

7. The method of claim 6 further comprising the steps of:

(e) illuminating the sample with photons emanating from the portable device to thereby produce Raman-scattered photons from a third set of multiple points in the sample wherein said third set of multiple points corresponds to a point in said second set of multiple points from which said Raman-scattered photons are characteristic of the presence of the agent; and

(f) simultaneously analyzing the Raman-scattered photons from said third set of multiple points in the sample using the portable device wherein said Raman-scattered photons from said third set of multiple points are characteristic of the presence or absence of the agent in the sample.

8. The method of claim 1 wherein at least three of the points in said first set of multiple points are collinear along a first line.

9. The method of claim 8 wherein at least three of the points in said first set of multiple points are collinear along a second line.

10. The method of claim 1 wherein at least four of the points in said first set of multiple points are equidistant from a point in the sample.

11. The method of claim 1 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 25% of the area in the field of view.

12. The method of claim 1 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 5% of the area in the field of view.

13. The method of claim 1 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 1% of the area in the field of view.

14. The method of claim 1 wherein the agent is a hazardous agent.

15. The method of claim 1 wherein the agent is a chemical agent.

16. The method of claim 1 wherein the agent is a biological toxin.

17. The method of claim 1 wherein the agent is a microorganism.

18. The method of claim 1 wherein the agent is a bacterium.

19. The method of claim 1 wherein the agent is a protozoan.

20. The method of claim 1 wherein the agent is a virus.

21. The method of claim 1 wherein the agent is a selected from the group consisting of:

Anthrax (Bacillus anthracis), protozoa, cryptosporidia, Escherichia coli, Plague (Yersinia pestis), Smallpox (variola major), Tularemia (Francisella tularensis), Brucellosis (Brucella species), Clostridium pertringens, Glanders (Burkholderia mallei), Melioidosis (Berkholderia pseudomallei), Psittacosis (Chlamydia psittaci), Q fever (Coxiella burnetii), Typhus fever (Rickettsia prowazekii), Vibrio (Vibrio cholerae), Giardia, Candida albicans, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Enterobacter aerogenes, Corynebacterium diphtheriae, Pseudomonas aeruginosa, Acinetobacter calcoaceticus, Klebsiella pneumoniae, Serratia marcescens, filoviruses, naviruses, alphaviruses, Salmonella, and Shigella.

22. The method of claim 1 wherein the Raman-scattered photons pass through a filter prior to analyzing the Raman-scattered photons for the presence or absence of the agent in the sample.

23. The method of claim 1 wherein the Raman-scattered photons pass through an interferometer prior to analyzing the Raman-scattered photons for the presence or absence of the agent in the sample.

24. The method of claim 1 wherein the Raman-scattered photons pass through a dispersive spectrometer prior to analyzing the Raman-scattered photons for the presence or absence of the agent in the sample.

25. The method of claim 1 wherein the area of one of the points in said first set of multiple points is at least twice as large as the area of another of the points in said first set of multiple points.

26. The method of claim 1 wherein the step of analyzing includes comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of the agent.

27. A method of identifying an agent in a sample, comprising the steps of:

(a) illuminating the sample with photons emanating from a portable device to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship;

(b) simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device; and

(c) comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of the agent.

28. A method of assessing viability of an agent in a sample, comprising the steps of:

(a) illuminating the sample with photons emanating from a portable device to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship;

(b) simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device; and

(c) comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of a viable or nonviable form of the agent.

29. A portable device for assessing occurrence of an agent in a sample, comprising:

(a) a portable device for illuminating the sample with photons to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship; and

(b) means for simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device wherein said Raman-scattered photons are characteristic of the presence or absence of the agent in the sample.

30. The device of claim 29 wherein the number of points in the first set of multiple points is three.

31. The device of claim 29 wherein the number of points in the first set of multiple points is greater than or equal to four and less than or equal to ten.

32. The device of claim 29 wherein the number of points in the first set of multiple points is greater than or equal to ten and less than or equal to fifty.

33. The device of claim 29 wherein the number of points in the first set of multiple points is greater than fifty.

34. The device of claim 29 wherein at least three of the points in said first set of multiple points are collinear along a first line.

35. The device of claim 29 wherein at least three of the points in said first set of multiple points are collinear along a second line.

36. The device of claim 29 wherein at least four of the points in said first set of multiple points are equidistant from a point in the sample.

37. The device of claim 29 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 25% of the area in the field of view.

38. The device of claim 29 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 5% of the area in the field of view.

39. The device of claim 29 wherein the sample is in a field of view of a microscope and the area covered by the points in said first set of multiple points is less than 1% of the area in the field of view.

40. The device of claim 29 wherein the agent is a hazardous agent.

41. The device of claim 29 wherein the agent is a chemical agent.

42. The device of claim 29 wherein the agent is a biological toxin.

43. The device of claim 29 wherein the agent is a microorganism.

44. The device of claim 29 wherein the agent is a bacterium.

45. The device of claim 29 wherein the agent is a protozoan.

46. The device of claim 29 wherein the agent is a virus.

47. The device of claim 29 wherein the agent is a selected from the group consisting of:

Anthrax (Bacillus anthracis), protozoa, cryptosporidia, Escherichia coli, Plague (Yersinia pestis), Smallpox (variola major), Tularemia (Francisella tularensis), Brucellosis (Brucella species), Clostridium pertringens, Glanders (Burkholderia mallei), Melioidosis (Berkholderia pseudomallei), Psittacosis (Chlamydia psittaci), Q fever (Coxiella burnetii), Typhus fever (Rickettsia prowazekii), Vibrio (Vibrio cholerae), Giardia, Candida albicans, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Enterobacter aerogenes, Corynebacterium diphtheriae, Pseudomonas aeruginosa, Acinetobacter calcoaceticus, Klebsiella pneumoniae, Serratia marcescens, filoviruses, naviruses, alphaviruses, Salmonella, and Shigella.

48. The device of claim 29 further comprising a filter.

49. The device of claim 48 wherein the filter is a tunable filter.

50. The device of claim 49 wherein the tunable filter is a liquid crystal tunable filter.

51. The device of claim 50 wherein the liquid crystal tunable filter is selected from the group consisting of:

Lyot liquid crystal tunable filter, Evans Split-Element liquid crystal tunable filter, Solc liquid crystal tunable filter, Ferroelectric liquid crystal tunable filter, and Fabry Perot liquid crystal tunable filter.

52. The device of claim 29 further comprising an interferometer.

53. The device of claim 52 wherein the interferometer is selected from the group consisting of:

Sagnac interferometers, Michelson interferometers, Twynam-Green interferometers, and Mach-Zehnder interferometers.

54. The device of claim 29 further comprising a fixed bandpass or a fixed band reject filter.

55. The device of claim 29 further comprising a polarizing filter.

56. The device of claim 29 further comprising a dispersive spectrometer.

57. The device of claim 29 wherein the area of one of the points in said first set of multiple points is at least twice as large as the area of another of the points in said first set of multiple points.

58. The device of claim 29 including means for comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of the agent.

59. A portable device for identifying an agent in a sample, comprising:

(a) a portable device for illuminating the sample with photons to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship;

(b) means for simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device wherein said Raman-scattered photons are characteristic of the presence or absence of the agent in the sample; and

(c) means for comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of the agent.

60. A portable device for assessing viability of an agent in a sample, comprising:

(a) a portable device for illuminating the sample with photons to thereby produce Raman-scattered photons from a first set of multiple points in the sample wherein the points of said first set of multiple points have a defined geometric relationship;

(b) means for simultaneously analyzing the Raman-scattered photons from said first set of multiple points in the sample using the portable device wherein said Raman-scattered photons are characteristic of the presence or absence of the agent in the sample; and

(c) means for comparing the Raman-scattered photons from said first set of multiple points in the sample with a known Raman scattering characteristic of a viable or nonviable form of the agent.