×

Microscale diffusion immunoassay utilizing multivalent reactants

  • US 7,550,267 B2
  • Filed: 09/14/2005
  • Issued: 06/23/2009
  • Est. Priority Date: 09/23/2004
  • Status: Active Grant
First Claim
Patent Images

1. A method for detecting the presence of analyte particles in an analyte fluid comprising:

  • (a) providing the analyte fluid comprising the analyte particles;

    (b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles;

    (c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel;

    (d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes;

    (e) allowing the analyte/binding particle complexes to cross-link to form cross-linked aggregates; and

    (f) detecting the presence of the analyte particles and the cross-linked aggregates,wherein;

    each of the binding particles is capable of binding with more than one analyte particle;

    each of the analyte particles is capable of binding with more than one binding particle;

    the non-aggregated analyte/binding particle complexes and the analyte particles are at least the same order of magnitude in size and have diffusion coefficients that are essentially the same; and

    the cross-linked aggregates have a diffusion coefficient that is at least two times greater than the diffusion coefficients of the non-aggregated analyte/binding particle complexes and the analyte particles.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×