Delta-8 desaturase and its use in making polyunsaturated fatty acids
First Claim
Patent Images
1. An isolated polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide having delta 8 desaturase activity, wherein the polypeptide has an amino acid sequence comprising SEQ ID NO;
2;
or,(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.
2 Assignments
0 Petitions
Accused Products
Abstract
Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast.
53 Citations
14 Claims
-
1. An isolated polynucleotide comprising:
-
(a) a nucleotide sequence encoding a polypeptide having delta 8 desaturase activity, wherein the polypeptide has an amino acid sequence comprising SEQ ID NO;
2;
or,(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14)
-
-
13. A method for producing at least one polyunsaturated fatty acid in a soybean cell comprising:
-
(a) transforming a soybean cell with a first recombinant DNA construct comprising SEQ ID NO;
2, operably linked to at least one regulatory sequence and at least one additional recombinant DNA construct comprising an isolated polynucleotide, operably linked to at least one regulatory sequence, encoding a polypeptide selected from the group consisting of a delta-4, a delta-5, delta-6, a delta-9, a delta-12, a delta-15, and a delta-17 desaturase, a delta-9 elongase, a C18 to C22 elongase and a C20 to C24 elongase;(b) regenerating a soybean plant from the transformed cell of step (a); and (c) selecting those seeds obtained from the plants of step (b) having an altered level of polyunsaturated fatty acids when compared to the level in seeds obtained from a nontransformed soybean plant.
-
Specification