Method and apparatus for three-dimensional spectrally encoded imaging

• US 7,551,293 B2
• Filed: 11/24/2004
• Issued: 06/23/2009
• Est. Priority Date: 11/28/2003
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Iftimia, Nicusor, Yelin, Dvir, Tearney, Guillermo J., Bouma, Brett E.
• Primary Examiner(s): Connolly; Patrick J
• Application Number:
  US10/997,789
• Publication Number:
  US 20050128488A1
• Time in Patent Office: 1,672 Days
• Field of Search: 356/479, 356/456, 356/497, 356/511, 356/521
• US Class Current: 356/497
• CPC Class Codes: A61B 1/042 characterised by a proximal camera, e.g. a CCD camera
• CPC Class Codes: A61B 5/1077 Measuring of profiles
• CPC Class Codes: G01B 11/2441 using interferometry
• CPC Class Codes: G01B 11/2509 Color coding
• CPC Class Codes: G01B 9/02027 Two or more interferometric channels or interferometers
• CPC Class Codes: G01B 9/02044 Imaging in the frequency domain, e.g. by using a spectrometer

Abstract

A method and apparatus for providing information associated with at least one portion of a sample can be provided. For example, at least one wavelength of electro-magnetic radiation provided on a sample can be encoded to determine at least one transverse location of the portion. A relative phase between at least one first electro-magnetic radiation electro-magnetic radiation being returned from a sample and at least one second electro-magnetic radiation returned from a reference can be obtained to determine at least one relative depth location of the portion. Further, the information of the portion can be provided based on the transverse location and the relative depth location. For example, the information can be depth information and/or three-dimensional information associated with the portion of the sample.

Claims

1. A method for providing information associated with at least one portion of a sample, comprising:

encoding at least two wavelengths of at least one particular electro-magnetic radiation provided on a sample, each of the at least two wavelengths being utilized to determine at least one respective transverse location of a plurality of transverse location of the at least one portion;

obtaining at least one relative phase between at least one first electro-magnetic radiation electro-magnetic radiation being returned from the sample and at least one second electro-magnetic radiation returned from a reference to determine at least one relative depth location of the at least one portion; and

providing the information of the at least one portion based on each of the transverse locations and the at least one relative depth location.

2. The method according to claim 1, further comprising:

transversely scanning the at least one first electro-magnetic radiation across the at least one portion of the sample in one dimension to obtain three-dimensional data based on the transverse locations.

3. The method according to claim 1, wherein the information is provided as a function of the two-dimensional data.

4. The method according to claim 3, wherein the information is a three-dimensional information for the at least one portion which is obtained based on the two-dimensional data.

5. The method according to claim 1, wherein the information is a three-dimensional information for the at least one portion.

6. The method according to claim 1, wherein the at least one first electro-magnetic radiation and the at least one second electro-magnetic radiation have a first path length, and wherein the at least one relative phase is determined by:

detecting a first interference signal produced by an interference between a phase of the first and second electro-magnetic radiations having the path length;

modifying a relative position of the reference to a second path length, and receiving at least one third electro-magnetic radiation from the sample and at least one fourth electro-magnetic radiation having a second path length;

detecting a second interference signal produced by an interference between a phase of the third and fourth electro-magnetic radiations having the second path length; and

processing the first and second interference signal to provide interference data.

7. The method according to claim 6, wherein the information is provided based on the interference information.

8. The method according to claim 7, wherein the information includes depth information of the at least one portion.

9. The method according to claim 8, wherein the depth information is determined by measuring the first interference signal.

10. The method according to claim 9, wherein the depth information is further determined by measuring the second interference signal.

11. The method according to claim 8, wherein the second and fourth electro-magnetic radiations have different phases.

12. The method according to claim 7, wherein the information includes height information of the at least one portion at a plurality of transverse locations on the at least one portion.

13. The method according to claim 1, further comprising:

effectuating discrete changes in a length of a path of the reference to generate the at least one relative phase.

14. The method according to claim 1, wherein the at least one location includes a plurality of locations.

15. The method according to claim 14, wherein each of the wavelengths is associated with a respective one of the locations.

16. The method according to claim 1, wherein the at least one particular electro-magnetic radiation is provided on at least two separate respective locations on the sample.

17. A method for obtaining three-dimensional information associated with a specimen, comprising:

receiving at least one first electro-magnetic radiation from a sample arm which includes the specimen based on a transversely spectrally encoded electro-magnetic radiation provided to the specimen;

receiving at least one second electro-magnetic radiation from a reference arm based on a first path length and a second path length thereof which are different from one another;

generating interference information based on the first and second electro-magnetic radiations; and

providing the three-dimensional information associated with the specimen as a function of the interference information.

18. The method according to claim 17, wherein the second path length is produced by moving a reflective surface in the reference arm from a position of the first path length of the reference arm.

19. The method according to claim 18, wherein the interference information is generated by combining and transversely spatially dispersing the first and second electro-magnetic radiations using a dispersive arrangement.

20. The method according to claim 19, further comprising providing the dispersed electro-magnetic radiation to an imaging system so as to generate the three-dimensional information.

21. The method according to claim 20, wherein the dispersed electro-magnetic radiation is provided onto a charge-coupled device.

22. An apparatus for providing three-dimensional imaging information associated with at least one portion of a sample, comprising:

at least one first arrangement configured to provide at least two wavelengths of at least one particular electro-magnetic radiation provided on the portion of the sample, and to determine, using each of the at least two wavelengths, at least one respective transverse location of a plurality of transverse location of the at least one portion;

at least one second arrangement configured to obtain a relative phase between at least one first electro-magnetic radiation electro-magnetic radiation being returned from a sample and at least one second electro-magnetic radiation returned from a reference to determine a relative depth location of the portion; and

at least one third arrangement configured to provide the information of the portion based on the transverse location and the relative depth location.

23. The apparatus according to claim 22, further comprising:

at least one fourth arrangement configured to transversely scan the at least one first electro-magnetic radiation across the at least one portion of the sample in one dimension to obtain three-dimensional data based on the transverse locations.

24. The apparatus according to claim 23, wherein the information is provided as a function of the two-dimensional data.

25. The apparatus according to claim 24, wherein the information is a three-dimensional information for the at least one portion which is obtained based on the two-dimensional data.

26. The apparatus according to claim 22, wherein the information is a three-dimensional information for the at least one portion.

27. The apparatus according to claim 22, further comprising a dispersive arrangement configured to combining and transversely spatially dispersing the first and second electro-magnetic radiations to generate the information.

28. The apparatus according to claim 22, further comprising:

at least one fifth arrangement configured to effectuate discrete changes in a length of a path of the reference to generate the at least one relative phase.

29. The apparatus according to claim 22, wherein the at least one location includes a plurality of locations.

30. The apparatus according to claim 29, wherein each of the wavelengths is associated with a respective one of the locations.

31. The apparatus according to claim 22, wherein the at least one particular electro-magnetic radiation is provided on at least two separate respective locations on the sample.

32. An apparatus for obtaining three-dimensional information associated with a specimen, comprising:

at least one first arrangement configured to receive;

at least one first electro-magnetic radiation from a sample arm which includes the specimen based on a transversely spectrally encoded electro-magnetic radiation provided to the specimen, and

at least one second electro-magnetic radiation from a reference arm based on a first path length and a second path length thereof which are different from one another;

at least one second arrangement configured to generate interference information based on the first and second electro-magnetic radiations; and

at least one third arrangement configured to provide the three-dimensional information associated with the specimen as a function of the interference information.

33. The apparatus according to claim 32, further comprising a dispersive arrangement configured to combining and transversely spatially dispersing the first and second electro-magnetic radiations to generate the three-dimensional information.

34. The apparatus according to claim 33, further comprising a charge-coupled device configured to receive the dispersed electro-magnetic radiation.