Assay implementation in a microfluidic format

• US 7,553,453 B2
• Filed: 12/29/2006
• Issued: 06/30/2009
• Est. Priority Date: 06/02/2000
• Status: Expired due to Term

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Padmanabhan, Aravind, Gu, Yuandong
• Primary Examiner(s): WALLENHORST, MAUREEN
• Application Number:
  US11/618,502
• Publication Number:
  US 20070190525A1
• Time in Patent Office: 914 Days
• Field of Search: 436/8, 436/10, 436/63, 436/66, 436/164, 436/165, 436/172, 436/174, 436/180, 422/61, 422/68.1, 422/73, 422/82.05, 422/82.08, 422/100, 435/5, 435/7.1, 435/7.22, 435/7.24, 435/7.25, 435/7.32, 435/29, 435/34, 435/287.2, 435/287.3, 435/288.3, 435/288.4, 435/288.7
• US Class Current: 422/537
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2200/146 Employing pressure sensors
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/012 Red blood cells
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/019 Biological contaminants; Fouling
• CPC Class Codes: G01N 2015/1486 : Counting the particles
• CPC Class Codes: G01N 2035/00158 : Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 2333/445 : Plasmodium
• CPC Class Codes: G01N 2333/70514 : CD4
• CPC Class Codes: G01N 2333/70589 : CD45
• CPC Class Codes: G01N 2800/26 : Infectious diseases, e.g. generalised sepsis
• CPC Class Codes: G01N 33/5094 : for blood cell populations red blood cells G01N33/80
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

An assay implementation in a microfluidic format in a cartridge relating to a point-of-care instrument platform for monitoring and diagnosing infectious diseases (e.g., AIDS and malaria). The platform may also provide a complete blood count. The instrument platform may hold the cartridge and a portion of an optical system for fluorescent and scattered light related analyses of blood sample in a flow channel of the cartridge.

Claims

1. A point of care analyzer comprising:

a portable cartridge holding instrument; and

a microfluidic cartridge insertable in the portable cartridge holding instrument, wherein the cartridge is credit-card sized; and

wherein;

the microfluidic cartridge comprises;

a cytometry flow channel having transparent windows;

an intersecting region having an output connected to the cytometry flow channel;

a sample channel connected to a first input of the intersecting region;

a sheath fluid channel connected to a second input of the intersecting region;

a sheath fluid reservoir connected to the sheath fluid channel; and

a waste reservoir connected to the cytometry flow channel;

the intersecting region is for hydrofocusing a sample having particles from the sample channel with a sheath fluid into a single file core of particles;

the cytometry flow channel is for conveying the single file of particles with the sheath fluid through the transparent windows of the cytometry flow channel and to the waste reservoir;

when the microfluidic cartridge is inserted in the portable cartridge holding instrument, the transparent windows are aligned with one or more light sources and one or more detectors in the portable cartridge holding instrument to form both a scattering optical channel and a fluorescence optical channel; and

the microfluidic cartridge is disposable upon at least a partial filling of the waste reservoir with particles from the cytometry flow channel.

2. The analyzer of claim 1, wherein the particles in the waste reservoir are regarded as biological waste.

3. The analyzer of claim 2, wherein the cartridge having the waste reservoir containing the biological waste is disposable in accordance with applicable health regulations.

4. The analyzer of claim 1, further comprising:

a second intersecting region having an output connected to the sample channel;

a blood reservoir having an output connected to the second intersecting region; and

a lysing reagent reservoir having an output connected to the second intersecting region.

5. The analyzer of claim 4, further comprising an input port connected to the blood reservoir.

6. The analyzer of claim 5, wherein:

the sample having particles is blood; and

the lysing reagent is for lysing red blood cells in the blood.

7. The analyzer of claim 6, wherein the lysing red blood cells occurs in the second intersecting region and/or the sample channel.

8. The analyzer of claim 7, wherein the lysing red cells in the second intersecting region and/or the sample channel comprises lysing-on-the-fly.

9. The analyzer of claim 1, further comprising:

a second intersecting region having an output connected to the sample channel;

a blood reservoir having an output connected to the second intersecting region; and

a sphering reagent reservoir having an output connected to the second intersecting region.

10. The analyzer of claim 9, wherein:

the sample having particles is blood; and

the sphering reagent is for sphering red blood cells in the blood.

11. The analyzer of claim 10, wherein the sphering red blood cells occurs in the second intersecting region and/or the sample channel.

12. The analyzer of claim 11, wherein the sphering red cells in the second intersecting region and/or the sample channel comprises sphering-on-the-fly.

13. The analyzer of claim 1, wherein a first optical circuit comprises the transparent windows of the cytometry flow channel, and at least one of the one or more light sources and at least one of the one or more detectors of the cartridge holding instrument aligned with the transparent windows.

14. The analyzer of claim 13, wherein a second optical circuit comprises the transparent windows of the cytometry flow channel, and at least one of the one or more light sources and at least one of the one or more detectors of the cartridge holding instrument aligned with the transparent windows.

15. The analyzer of claim 14, wherein the sample having particles is blood:

the first optical circuit comprises at least one scattering optical channel for determining a count and/or properties of particles of the blood sample; and

the second optical circuit comprises at least one fluorescent optical channel for detecting and identifying markers on particles of the blood sample for an immunoassay.

16. The analyzer of claim 15, wherein the scattering optical channel is for detecting light scattered by particles of the sample at various angles.

17. The analyzer of claim 16, wherein light scattered by the particles is for the determining a count and/or properties of particles of the blood sample.

18. The analyzer of claim 15, wherein the immunoassay is for detecting particles of the blood sample that are at least one of a group of CD4, CD45, CDX, CDXX, p. falciparum, p. vivax, and various other pathogens.

19. The analyzer of claim 18, wherein the pathogens can effect AIDS, malaria, HIV, or TB.

20. The analyzer of claim 18, wherein the immunoassay comprises:

incubating and tagging particles with fluorescent antibodies;

impinging the particles with light;

detecting fluorescence emitted from the tagged particles;

detecting light scattered by the particles; and

determining CDXX and/or pathogen data from fluorescence emitted from the tagged particles and/or light scattered by the particles.

21. The system of claim 1, wherein the cartridge is a microfluidic cartridge comprising plastic type and/or glass type materials having autofluoresence properties as good or better than glass at 488 nm.

22. The system of claim 1, wherein the cartridge comprises a cyclic olefin copolymer based plastic material structure.

23. The system of claim 1, wherein the cartridge is a laminated structure with etched channels for microfluidic circuits.

24. The system of claim 1, wherein the cartridge has a molded structure containing channels for microfluidic circuits.

25. A point of care analyzer comprising:

a portable cartridge holding instrument; and

a microfluidic cartridge insertable in the portable cartridge holding instrument, wherein the cartridge is credit-card sized; and

wherein;

the microfluidic cartridge comprises;

a first cytometry flow channel having transparent windows;

a first intersecting region having an output connected to the first cytometry flow channel;

a first sample channel connected to a first input of the first intersecting region;

a first sheath fluid channel connected to a second input of the first intersecting region;

a first sheath fluid reservoir connected to the first sheath fluid channel; and

a first waste reservoir connected to the an output of the first cytometry flow channel;

a second intersecting region having an output connected to the first sample channel;

a first blood reservoir having an output connected to the second intersecting region;

a lysing reagent reservoir having an output connected to the second intersecting region

a second cytometry flow channel having transparent windows;

a third intersecting region having an output connected to the second cytometry flow channel;

a second sample channel connected to a first input of the third intersecting region;

a second sheath fluid channel connected to a second input of the third intersecting region;

a second sheath fluid reservoir connected to the second sheath fluid channel;

a second waste reservoir connected to an output of the second cytometry flow channel;

a fourth intersecting region having an output connected to the second sample channel;

a second blood reservoir having an output connected to the fourth intersecting region; and

a sphering reagent reservoir having an output connected to the fourth intersecting region, wherein when the microfluidic cartridge is inserted in the portable cartridge holding instrument, the transparent windows are aligned with one or more light sources and one or more detectors in the portable cartridge holding instrument to form both a scattering optical channel and a fluorescence optical channel.

26. The analyzer of claim 25, wherein:

the first intersecting region is for hydrofocusing a sample having particles from the first sample channel with a sheath fluid into a first single file core of particles;

the first cytometry flow channel is for conveying the first single file of particles with the sheath fluid by the transparent windows of the first cytometry flow channel and then to the first waste reservoir;

the third intersecting region is for hydrofocusing a sample having particles from the second sample channel with a sheath fluid into a second single file core of particles; and

the second cytometry flow channel is for conveying the second single file of particles with the sheath fluid by the transparent windows of the second cytometry flow channel and then to the second waste reservoir.

27. The analyzer of claim 26, wherein:

when the microfluidic cartridge is inserted in the portable cartridge holding instrument, the transparent windows of the cytometry flow channels are aligned with one or more light sources and one or more detectors in the portable cartridge holding instrument; and

the microfluidic cartridge is disposable upon a partial filling of the first waste reservoir with particles from the first cytometry flow channel.

28. The analyzer of claim 27, wherein particles from a cytometry flow channel to the waste reservoir are to be regarded as biological waste.

29. The analyzer of claim 28, wherein the cartridge containing the waste in the waste reservoir is disposed in accordance with applicable health regulations.

30. The analyzer of claim 27, wherein a first optical circuit comprises the transparent windows of a cytometry flow channel, and at least one of the one or more light sources and at least one of the one or more detectors of the cartridge holding instrument aligned with the transparent windows.

31. The analyzer of claim 30, wherein a second optical circuit comprises the transparent windows of a cytometry flow channel, and at least one of the one or more light sources and at least one of the one or more detectors of the portable cartridge holding instrument aligned with the transparent windows.

32. The analyzer of claim 31, wherein the first and second sample channels transport a blood sample there through:

the first optical circuit comprises at least one scattering optical channel for determining a count and/or properties of particles of the blood sample;

the second optical circuit comprises at least one fluorescent optical channel for detecting and identifying markers on particles of the blood sample for an immunoassay;

the immunoassay is for detecting particles of the blood sample that are at least one of a group of CD4, CD45, CDX, CDXX, p. falciparum, p. vivax, and various other pathogens; and

light scattered by the particles is for the determining a count and/or properties of particles of the blood sample.

33. A point of care analyzer comprising:

a portable cartridge holding instrument; and

a microfluidic cartridge insertable in the portable cartridge holding instrument, wherein the cartridge is credit-card sized; and

wherein;

the microfluidic cartridge comprises;

a cytometry flow channel having transparent windows;

an intersecting region having an output connected to the cytometry flow channel;

a sample channel connected to a first input of the intersecting region;

a sheath fluid channel connected to a second input of the intersecting region;

a sheath fluid reservoir connected to the sheath fluid channel; and

a waste reservoir connected to the cytometry flow channel;

the intersecting region is for hydrofocusing a sample having particles from the sample channel with a sheath fluid into a single file core of particles;

the cytometry flow channel is for conveying the single file of particles with the sheath fluid through the transparent windows of the cytometry flow channel and to the waste reservoir;

when the microfluidic cartridge is inserted in the portable cartridge holding instrument, the transparent windows are aligned with one or more light sources and one or more detectors in the portable cartridge holding instrument to form both a scattering optical channel and a fluorescence optical channel; and

the microfluidic cartridge is disposable after an entry of any particles and/or sheath fluid from the cytometry flow channel to the waste reservoir.

34. The system of claim 33, wherein the cartridge comprises a cyclic olefin copolymer based plastic material structure.