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Assay implementation in a microfluidic format

  • US 7,553,453 B2
  • Filed: 12/29/2006
  • Issued: 06/30/2009
  • Est. Priority Date: 06/02/2000
  • Status: Expired due to Term
First Claim
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1. A point of care analyzer comprising:

  • a portable cartridge holding instrument; and

    a microfluidic cartridge insertable in the portable cartridge holding instrument, wherein the cartridge is credit-card sized; and

    wherein;

    the microfluidic cartridge comprises;

    a cytometry flow channel having transparent windows;

    an intersecting region having an output connected to the cytometry flow channel;

    a sample channel connected to a first input of the intersecting region;

    a sheath fluid channel connected to a second input of the intersecting region;

    a sheath fluid reservoir connected to the sheath fluid channel; and

    a waste reservoir connected to the cytometry flow channel;

    the intersecting region is for hydrofocusing a sample having particles from the sample channel with a sheath fluid into a single file core of particles;

    the cytometry flow channel is for conveying the single file of particles with the sheath fluid through the transparent windows of the cytometry flow channel and to the waste reservoir;

    when the microfluidic cartridge is inserted in the portable cartridge holding instrument, the transparent windows are aligned with one or more light sources and one or more detectors in the portable cartridge holding instrument to form both a scattering optical channel and a fluorescence optical channel; and

    the microfluidic cartridge is disposable upon at least a partial filling of the waste reservoir with particles from the cytometry flow channel.

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