Specific coupling reaction measuring method
First Claim
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1. A specific coupling reaction measuring method for measuring a plurality of subject substances in a sample, comprising the steps of:
- (a) constructing a reaction system including said sample and a specific coupling substance for specifically coupling with a first subject substance;
(b) measuring an optical characteristic of said reaction system;
(c) adding, to said reaction system, a magnetic complex including a magnetic particle and a substance capable of coupling with an agglutination complex including said first subject substance and said specific coupling substance;
(d) removing, by utilizing magnetic force, said agglutination complex coupled with said magnetic complex from said reaction system;
(e) adding, to said reaction system, another specific coupling substance for specifically coupling with another subject substance; and
(f) measuring an optical characteristic of said reaction system after the step (e).
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Abstract
A specific coupling reaction measuring method of this invention for measuring a content of a subject substance in a sample includes the steps of (a) constructing a reaction system including the sample and a magnetic particle on which a specific coupling substance for specifically coupling with the subject substance is immobilized; (b) measuring an optical characteristic of the reaction system; and (c) removing, from the reaction system, an agglutination complex including the subject substance, the specific coupling substance and the magnetic particle by utilizing magnetic force.
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Citations
8 Claims
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1. A specific coupling reaction measuring method for measuring a plurality of subject substances in a sample, comprising the steps of:
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(a) constructing a reaction system including said sample and a specific coupling substance for specifically coupling with a first subject substance; (b) measuring an optical characteristic of said reaction system; (c) adding, to said reaction system, a magnetic complex including a magnetic particle and a substance capable of coupling with an agglutination complex including said first subject substance and said specific coupling substance; (d) removing, by utilizing magnetic force, said agglutination complex coupled with said magnetic complex from said reaction system; (e) adding, to said reaction system, another specific coupling substance for specifically coupling with another subject substance; and (f) measuring an optical characteristic of said reaction system after the step (e). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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Specification