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Specific coupling reaction measuring method

  • US 7,553,676 B2
  • Filed: 08/21/2006
  • Issued: 06/30/2009
  • Est. Priority Date: 11/12/2002
  • Status: Active Grant
First Claim
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1. A specific coupling reaction measuring method for measuring a plurality of subject substances in a sample, comprising the steps of:

  • (a) constructing a reaction system including said sample and a specific coupling substance for specifically coupling with a first subject substance;

    (b) measuring an optical characteristic of said reaction system;

    (c) adding, to said reaction system, a magnetic complex including a magnetic particle and a substance capable of coupling with an agglutination complex including said first subject substance and said specific coupling substance;

    (d) removing, by utilizing magnetic force, said agglutination complex coupled with said magnetic complex from said reaction system;

    (e) adding, to said reaction system, another specific coupling substance for specifically coupling with another subject substance; and

    (f) measuring an optical characteristic of said reaction system after the step (e).

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