Method for gene identification signature (GIS) analysis

• US 7,553,947 B2
• Filed: 12/13/2006
• Issued: 06/30/2009
• Est. Priority Date: 09/17/2003
• Status: Expired due to Fees

• Current Assignee: Agency For Science Technology and Research
• Original Assignee: Agency For Science Technology and Research
• Inventors: Ng, Patrick, Wei, Chialin, Ruan, Yijun
• Primary Examiner(s): Bausch; Sarae
• Assistant Examiner(s): Pohnert; Steven C
• Application Number:
  US11/610,118
• Publication Number:
  US 20070161024A1
• Time in Patent Office: 930 Days
• Field of Search: 435/320.1, 536/24.2, 536/23.1
• US Class Current: 536/23.1
• CPC Class Codes: C12N 15/1065 Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
• CPC Class Codes: C12N 15/1093 General methods of preparing gene libraries, not provided for in other subgroups
• CPC Class Codes: C12N 15/1096 cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/6855 Ligating adaptors
• CPC Class Codes: C12Q 2521/313 Type II endonucleases, i.e. cutting outside recognition site
• CPC Class Codes: C12Q 2525/131 incorporating a restriction site
• CPC Class Codes: C12Q 2525/191 incorporating an adaptor

Abstract

A method of identifying at least a nucleic acid molecule fragment to which a protein of interest binds, comprising: (i) preparing at least one nucleic acid molecule fragment to which a protein binds; (ii) isolating the 5′ terminus and the 3′ terminus of the nucleic acid fragment(s) and linking the 5′ terminus and 3′ terminus to create the at least one ditag; (iii) sequencing the ditag; and (iv) mapping the ditag sequence(s) to the genome.

Claims

1. A vector having the nucleotide sequence of SEQ ID NO:

22.