Reversible terminator nucleotides and methods of use

• US 7,553,949 B2
• Filed: 06/14/2007
• Issued: 06/30/2009
• Est. Priority Date: 06/30/2006
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Zon, Gerald, Lee, Linda G.
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/763,405
• Publication Number:
  US 20080050780A1
• Time in Patent Office: 747 Days
• Field of Search: 536/23.1, 536/24.3, 536/26.6, 435/6, 435/91.1
• US Class Current: 536/23.1
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2521/525 Phosphatase
• CPC Class Codes: C12Q 2525/101 incorporating non-naturally occurring nucleotides, e.g. inosine

Abstract

Disclosed herein a reversible terminator nucleotides and methods of use.

Claims

1. A nucleoside comprising a base and a sugar in which a phosphate moiety label is attached to said base and a phosphate moiety protecting group is attached to said sugar, wherein said phosphate moiety label and said phosphate moiety protecting group are detachable from said nucleoside by an enzyme.

2. A nucleoside comprising a base and a sugar in which a SO₃ moiety label is attached to said base and a SO₃ moiety protecting group is attached to said sugar, wherein said SO₃ moiety label and said SO₃ moiety protecting group are detachable from said nucleoside by an enzyme.

3. A nucleotide comprising a base and a sugar in which a phosphate moiety label is attached to said base and a phosphate moiety protecting group is attached to said sugar, wherein said phosphate moiety label and said phosphate moiety protecting group are detachable from said nucleotide by an enzyme.

4. A nucleotide comprising a base and a sugar in which a SO₃ moiety label is attached to said base and a SO₃ moiety protecting group is attached to said sugar, wherein said SO₃ moiety label and said SO₃ moiety protecting group are detachable from said nucleotide by an enzyme.

5. A nucleotide comprising a base and a sugar in which a label is attached to said base and a protecting group is attached to said sugar, wherein said label and said protecting group are detachable from said nucleotide by a phosphatase enzyme.

6. A nucleotide comprising a base and a sugar in which a label is attached to said base and a protecting group is attached to said sugar, wherein said label and said protecting group are detachable from said nucleotide by a shrimp alkaline phosphatase enzyme.