Microscope with a viewing direction perpendicular to the illumination direction
First Claim
1. A microscope having at least one illumination beam path and at least one detection beam path, characterized in thateach illumination beam path is provided with a focusing arrangement for producing a linear object illumination region which extends in a direction of an illumination axis of the illumination beam path,a detection direction of the at least one detection beam path is approximately orthogonal to the linear object illumination region, andat least one mobile arrangement is provided for producing a relative movement between the linear object illumination region and an object to be studied,wherein the linear object illumination region is essentially limited to the direction of the illumination axis.
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Accused Products
Abstract
The invention relates to a microscope, in which a layer of the sample is illuminated by a thin strip of light (11) and the sample is viewed (5) perpendicular to the plane of the strip of light. The depth of the strip of light (11) thus essentially determines the depth of focus of the system. To record the image, the object (4) is displaced through the strip of light (11), which remains fixed in relation to the detector (8), and fluorescent and/or diffused light is captured by a planar detector. Objects (4) that absorb or diffuse a large amount of light are viewed from several spatial directions. The three-dimensional images, which are captured from each direction can be combined retrospectively to form one image, in which the data is weighted according to its resolution. The resolution of the combined image is then dominated by the lateral resolution of the individual images.
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Citations
8 Claims
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1. A microscope having at least one illumination beam path and at least one detection beam path, characterized in that
each illumination beam path is provided with a focusing arrangement for producing a linear object illumination region which extends in a direction of an illumination axis of the illumination beam path, a detection direction of the at least one detection beam path is approximately orthogonal to the linear object illumination region, and at least one mobile arrangement is provided for producing a relative movement between the linear object illumination region and an object to be studied, wherein the linear object illumination region is essentially limited to the direction of the illumination axis.
Specification