Electrochemical sensor strip and manufacturing method thereof

• US 7,556,724 B2
• Filed: 02/10/2005
• Issued: 07/07/2009
• Est. Priority Date: 02/10/2005
• Status: Active Grant

• Current Assignee: Bionime Corporation
• Original Assignee: Bionime Corporation
• Inventors: Huang, Chun-Mu
• Primary Examiner(s): Olsen; Kaj K
• Application Number:
  US11/054,894
• Publication Number:
  US 20060175199A1
• Time in Patent Office: 1,608 Days
• Field of Search: 204/403.01, 204/403.02, 204/403.03, 156/60
• US Class Current: 204/403.02
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: Y10T 156/10 Methods of surface bonding and/or assembly therefor

Abstract

An electrochemical sensor strip is provided. The electrochemical sensor strip includes an insulating substrate having a reaction concavity integrally formed thereon, a cover, an electrochemical reaction layer, and an electrode device. The reaction concavity is peripherally and entirely enclosed by the insulating substrate. The cover partially covers on the reaction concavity by leaving an opening on the reaction concavity. The electrochemical reaction layer is located in the reaction concavity, and the electrode device is located in the reaction concavity for transmitting a signal resulting from an electrochemical reaction performed in the electrochemical reaction layer.

Claims

1. An electrochemical sensor strip, comprising:

an insulating substrate having a reaction concavity integrally formed with said insulating substrate and located thereon and having a periphery entirely enclosed by said insulating substrate, wherein said reaction concavity has at least one hole at a bottom thereof;

a cover partially covering on said reaction concavity for leaving an opening on said reaction concavity;

an electrochemical reaction layer located in said reaction concavity; and

an electrode device located in said reaction concavity, embedded into said at least one hole and having a first end contacted with said electrochemical reaction layer and a second end contacted with a measuring device for transmitting a signal resulting from an electrochemical reaction performed in said electrochemical reaction layer to said measuring device, wherein said first and second ends are opposite to each other.

2. The electrochemical sensor strip according to claim 1, wherein a distance between a side of said reaction concavity and an edge of said insulating substrate is in a range from 0.1 mm to 3.0 mm.

3. The electrochemical sensor strip according to claim 2, wherein said electrode device comprises plural electrodes selected from a group consisting of a counter electrode, a working electrode, a reference electrode and a detecting electrode.

4. The electrochemical sensor strip according to claim 3, wherein each of said plural electrodes comprises a metal and a thin film.

5. The electrochemical sensor strip according to claim 4, wherein said metal is one selected from a group consisting of a copper, a titanium, a nickel, a gold, a platinum, a rhodium, a palladium, a ruthenium, a silver, a chromium, an iron, an aluminum, an iridium and an alloy thereof.

6. The electrochemical sensor strip according to claim 4, wherein said metal is one selected from a group consisting of a brass, an oxygen-free copper, a bronze, a phosphorized copper, a nickel silver copper and a beryllium copper.

7. The electrochemical sensor strip according to claim 4, wherein said thin film is made of one selected from a group consisting of a copper, a titanium, a nickel, a gold, a platinum, a rhodium, a palladium, a ruthenium, a silver, a chromium, an iron, an aluminum, an iridium and an alloy thereof.

8. The electrochemical sensor strip according to claim 3, wherein each of said plural electrodes comprises a carbon body and a thin film.

9. The electrochemical sensor strip according to claim 1, wherein said electrochemical reaction layer further comprises a chemical agent for reacting with an analyte of a sample to generate said signal.

10. The electrochemical sensor strip according to claim 9, wherein said sample is added into said reaction concavity via said opening.

11. The electrochemical sensor strip according to claim 1, wherein said insulating substrate is made of one selected from a group consisting of a polyvinyl chloride (PVC), a polypropylene (PP), a polycarbonate (PC), a polybutylene terephthalate (PBT), a polyethylene terephthalate (PET), a modified polyphenylene oxide (PPO) and an acrylonitrile butadiene styrene (ABS).

12. The electrochemical sensor strip according to claim 1, wherein said cover further comprises an air hole.

13. The electrochemical sensor strip according to claim 1, wherein a size of said opening is in a range from 0.1 mm to 3.0 mm.

14. A method for manufacturing an electrochemical sensor strip, comprising steps of:

a) providing an insulating piece having a reaction concavity integrally formed thereon and having a periphery entirely enclosed by said insulating piece, wherein said reaction concavity has at least one hole at a bottom thereof;

b) embedding at least an electrode device having an upper end and a lower end into said at least one hole to make said lower end contact to a measuring device;

c) forming a reaction layer contacted to said upper end in said reaction concavity; and

d) partially covering said reaction concavity to leave an opening on said reaction concavity.

15. The method according to claim 14, wherein said reaction layer is formed by applying a chemical reagent in said reaction concavity.

16. An electrochemical sensor strip, comprising:

an insulating substrate having a reaction concavity integrally formed thereon, wherein said reaction concavity comprises a first area, a second area and at least one hole at a bottom thereof;

a cover covering on said reaction concavity for forming an opening;

an electrochemical reaction layer located in said reaction concavity; and

an electrode device located in said reaction concavity, configured in said at least one hole and having a first surface contacted to said electrochemical reaction layer and a second surface contacted to an external device for transmitting a signal resulting from an electrochemical reaction performed in said electrochemical reaction layer to said external device,

wherein said second area is adjacent to said opening and said first and second surfaces are opposite to each other.

17. The electrochemical sensor strip according to claim 16, wherein said second area comprises a width ranged from 0.1 to 2.0 mm.

18. The electrochemical sensor strip according to claim 16, wherein said electrode device comprises plural electrodes selected from a group consisting of a counter electrode, a working electrode, a reference electrode and a detecting electrode, and each of said electrodes comprises a metal and a thin film.

19. The electrochemical sensor strip according to claim 16, wherein said cover further comprises an air hole.

20. The electrochemical sensor strip according to claim 16, wherein an edge portion of said insulating substrate is uneven.

21. The electrochemical sensor strip according to claim 16, wherein said second area has a second coarseness lower than a first coarseness of said first area.