Method for detecting a structured target
First Claim
1. A method for detecting an RNA analyte, the method comprising:
- (a) contacting a sample with a probe molecule under conditions permitting the probe to form a detectable probe;
analyte hybrid containing at least one 2′
-O-methyl ribonucleotide, wherein the probe binds to a double-stranded region of the analyte, and wherein the probe does not form a detectable probe;
non-analyte hybrid with non-target nucleic acid present in the sample; and
(b) determining whether the probe;
analyte hybrid has formed as an indication of the presence or absence of a target nucleic acid in the sample.
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Abstract
The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
56 Citations
25 Claims
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1. A method for detecting an RNA analyte, the method comprising:
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(a) contacting a sample with a probe molecule under conditions permitting the probe to form a detectable probe;
analyte hybrid containing at least one 2′
-O-methyl ribonucleotide, wherein the probe binds to a double-stranded region of the analyte, and wherein the probe does not form a detectable probe;
non-analyte hybrid with non-target nucleic acid present in the sample; and(b) determining whether the probe;
analyte hybrid has formed as an indication of the presence or absence of a target nucleic acid in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification