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Method for detecting a structured target

  • US 7,563,579 B2
  • Filed: 10/26/2007
  • Issued: 07/21/2009
  • Est. Priority Date: 07/16/1996
  • Status: Expired due to Fees
First Claim
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1. A method for detecting an RNA analyte, the method comprising:

  • (a) contacting a sample with a probe molecule under conditions permitting the probe to form a detectable probe;

    analyte hybrid containing at least one 2′

    -O-methyl ribonucleotide, wherein the probe binds to a double-stranded region of the analyte, and wherein the probe does not form a detectable probe;

    non-analyte hybrid with non-target nucleic acid present in the sample; and

    (b) determining whether the probe;

    analyte hybrid has formed as an indication of the presence or absence of a target nucleic acid in the sample.

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