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Microarray synthesis and assembly of gene-length polynucleotides

  • US 7,563,600 B2
  • Filed: 09/12/2002
  • Issued: 07/21/2009
  • Est. Priority Date: 09/12/2002
  • Status: Expired due to Term
First Claim
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1. A composition for the manufacture of a polynucleotide sequence comprising:

  • a first oligonucleotide comprising at its 5′

    end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 3′

    end of another oligonucleotide in the composition;

    a second oligonucleotide comprising at its 3′

    end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 5′

    end of another oligonucleotide in the composition; and

    a plurality of oligonucleotides wherein each oligonucleotide of the plurality comprises a first sequence region of about 10 to about 50 bases at its 5′

    end that is the same as a sequence region at the 3′

    end of another oligonucleotide in the composition and a second distinct sequence region of about 10 to about 50 bases at its 3′

    end that is the same as a sequence region at the 5′

    end of a different oligonucleotide in the composition;

    wherein the oligonucleotides of the composition are attached to a solid or porous surface and wherein the oligonucleotides of the composition together comprise the polynucleotide sequence.

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