Dynamic imaging of biological cells and other subjects

• US 7,564,546 B2
• Filed: 06/30/2005
• Issued: 07/21/2009
• Est. Priority Date: 06/30/2004
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Tuschel, David, Treado, Patrick J., Voigt, Thomas C., Maier, John S.
• Primary Examiner(s): Evans; F. L
• Application Number:
  US10/556,038
• Publication Number:
  US 20070182959A1
• Time in Patent Office: 1,482 Days
• Field of Search: 356/301
• US Class Current: 356/301
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01J 3/32 Investigating bands of a spectrum in sequence by a single detector
• CPC Class Codes: G01J 3/36 Investigating two or more bands of a spectrum by separate detectors
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 2021/656 Raman microprobe
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons

Abstract

The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

Claims

1. A method of cellular imaging to assess an effect of a drug on a cell, the method comprising:

(a) illuminating at least a portion of a cell with substantially monochromatic photons;

(b) detecting Raman-shifted photons scattered from the illuminated portion to thereby form an image corresponding to Raman-shifted photons collected substantially simultaneously from a plurality of different spatial locations of the cell;

(c) at each of the plurality of different spatial locations of the cell represented by the image, assessing the Raman-shifted photons at a plurality of Raman shift (RS) values characteristic of a pre-selected drug to thereby determine a first state of the cell;

(d) after a time period following a first iteration of steps (a)-(c), repeating steps (a)-(c) to thereby determine a second state of the cell; and

(e) comparing the first and second state of the cell to thereby assess an effect of the drug on the cell over the time period.

2. The method of claim 1 further comprising:

(f) assessing the Raman-shifted photons at a plurality of Raman shift (RS) values characteristic of a pre-selected component wherein said pre-selected component is selected from the group consisting of;

a metabolite of a drug, a cellular constituent, and combinations thereof.

3. The method of claim 1 wherein said cell is a living cell.

4. The method of claim 1 wherein said cell is a quiescent cell.

5. The method of claim 1 wherein said cell is a dead cell.

6. The method of claim 2 wherein said cellular constituent is selected from the group consisting of:

a particular protein, an organelle, a protein complex, a compartment, a membrane, and combinations thereof.

7. A method of cellular imaging to assess an effect of a drug on a cell, the method comprising:

(a) illuminating at least a portion of a cell with substantially monochromatic photons;

(b) filtering Raman-shifted photons scattered from the illuminated portion to thereby form a filtered image;

(c) collecting a first plurality of filtered images of the illuminated portion, wherein each filtered image corresponds to a different wavelength range of photons passed by the filter, and each filtered image corresponds to Raman-shifted photons collected substantially simultaneously from a plurality of different spatial locations of the cell;

(d) after a time period following a first iteration of steps (a)-(c), repeating steps (a)-(c) to collect a second plurality of filtered images; and

(e) comparing the first and second plurality of filtered images to thereby assess an effect of the drug on the cell over the time period.

8. The method of claim 7 further comprising:

(f) assessing the Raman-shifted photons at a plurality of Raman shift (RS) values characteristic of a pro-selected component wherein said pro-selected component is selected from the group consisting of;

a metabolite of a drug, a cellular constituent, and combinations thereof.

9. The method of claim 7 wherein said cell is a living cell.

10. The method of claim 7 wherein said cell is a quiescent cell.

11. The method of claim 7 wherein said cell is a dead cell.

12. The method of claim 8, wherein said cellular constituent is selected from the group consisting of:

a particular protein, an organelle, a protein complex, a compartment, a membrane, and combinations thereof.

13. The method of claim 7 wherein said Raman-shifted photons arc filtered using a filter selected from the group consisting of:

a tunable filter, a liquid crystal tunable filter. an acousto-optical tunable filter, an electro-optical tunable filter, and a mechanical tunable filter.