Albumin fusion proteins
First Claim
1. An expression vector comprising a promoter and termination region operably associated with a nucleic acid molecule encoding a leader sequence and an albumin fusion protein comprising two tandemly oriented Glucagon-Like-Peptide 1 (GLP-1) polypeptides fused to albumin or a fragment or variant of albumin, wherein said leader sequence is a modified HSA (human serum albumin)/Kex2 leader sequence having the amino acid sequence of SEQ ID NO:
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Accused Products
Abstract
The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention.
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Citations
10 Claims
- 1. An expression vector comprising a promoter and termination region operably associated with a nucleic acid molecule encoding a leader sequence and an albumin fusion protein comprising two tandemly oriented Glucagon-Like-Peptide 1 (GLP-1) polypeptides fused to albumin or a fragment or variant of albumin, wherein said leader sequence is a modified HSA (human serum albumin)/Kex2 leader sequence having the amino acid sequence of SEQ ID NO:
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2. The expression vector of claim wherein said promoter is selected from:
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(a) a Met25 promoter; (b) a cbh1 promoter; (c) a cysD promoter from Aspergillus nidulans; (d) a modified cbh1 promoter; (e) an alpha-mating factor promoter; and (f) a PRB1 promoter.
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Specification