Methods and devices for transporting and concentrating an analyte present in a sample

• US 7,569,398 B2
• Filed: 11/27/2001
• Issued: 08/04/2009
• Est. Priority Date: 11/29/2000
• Status: Expired due to Fees

• Current Assignee: Commissariat a L'Energie Atomique (AES Corporation), Biomerieux B.V. (Compagnie Merieux Alliance SAS)
• Original Assignee: Commissariat a L'Energie Atomique (AES Corporation)
• Inventors: Pouteau, Patrick, Ginot, Frederic, Caillat, Patrice, Puget, Pierre
• Primary Examiner(s): Shibuya; Mark L
• Assistant Examiner(s): Do; Pensee T
• Application Number:
  US10/416,207
• Publication Number:
  US 20040023273A1
• Time in Patent Office: 2,807 Days
• Field of Search: 436/518, 436/523, 436/524, 436/526, 436/534, 436/541, 436/540
• US Class Current: 436/518
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical applications

Abstract

The present invention relates to a method of transporting an analyte present in a sample, to a method of concentrating an analyte present in a sample, and to a device for implementing these methods. In the method of transporting an analyte present in a sample of the present invention, a solution A in which the analyte is attached to magnetic particles is prepared from the sample; the solution A is introduced into a first container connected via a bottleneck to a second container; and the analyte attached to the magnetic particles is moved, by means of a magnetic system, from the first container to the second container via the bottleneck, the second container being filled with all or part of the solution A and/or with another solution.

Claims

1. A method of concentrating an analyte present in a sample, comprising the following steps:

providing a solution A in which the analyte is attached to magnetic particles, by contacting the magnetic particles with the sample, in a first container of volume α

connected via a bottleneck to a second container of volume β

, the volume β

being smaller than the volume α

,

moving the analyte attached to the magnetic particles, by means of a magnetic system, from the first container to the bottleneck,

releasing the analyte attached to the magnetic particles from said particles in the bottleneck,

moving the magnetic particles released from the analyte from the bottleneck to said first container by means of a magnetic system, and

transporting the analyte from the bottleneck to the second container by movement of the solution A.

2. The method as claimed in claim 1, in which the analyte is released from the magnetic particles by modification of the physical or chemical conditions.

3. The method as claimed in claim 1, in which an agent for immobilizing the analyte is attached to all or part of at least one wall of the second container or of any solid support present in said second container.

4. The method as claimed in claim 1, in which the bottleneck is in the form of a capillary.

5. The method as claimed in claim 1, wherein the first container, the bottleneck and the second container are hollowed-out cavities in a single piece of material.

6. A method of concentrating an analyte present in a sample, comprising the following steps:

providing a solution A in which the analyte is attached to magnetic particles, by contacting the magnetic particles with the sample,

introducing the solution A into a first container of volume α

connected via a bottleneck to a second container of volume β

, the volume β

being smaller than the volume α

,

moving the analyte attached to the magnetic particles, by means of a magnetic system, from the first container to the bottleneck,

releasing the analyte attached to the magnetic particles from said particles in the bottleneck,

moving the magnetic particles released from the analyte from the bottleneck to said first container by means of a magnetic system, and

transporting the analyte from the bottleneck to the second container by movement of the solution A.

7. The method as claimed in claim 6, in which the analyte is released from the magnetic particles by modification of the physical or chemical conditions.

8. The method as claimed in claim 6, in which an agent for immobilizing the analyte is attached to all or part of at least one wall of the second container or of any solid support present in said second container.

9. The method as claimed in claim 6, in which the bottleneck is in the form of a capillary.

10. The method as claimed in claim 6, wherein the first container, the bottleneck and the second container are hollowed-out cavities in a single piece of material.