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Ligand/binding partner bio-labeling systems

  • US 7,575,866 B2
  • Filed: 09/01/2005
  • Issued: 08/18/2009
  • Est. Priority Date: 03/03/2003
  • Status: Active Grant
First Claim
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1. A method of detecting a target protein in a mammalian host cell, wherein the mammalian host cell expresses an endogenous functional dihydrofolate reductase gene, comprising:

  • (a) preparing a nucleic acid construct comprising a promoter element operatively linked to a nucleic acid encoding the target protein and a nucleic acid encoding a bacterial dihydrofolate reductase;

    (b) introducing the nucleic acid construct into a host cell;

    (c) culturing the host cell under conditions such that a target protein/dihydrofolate reductase fusion protein is expressed;

    (d) introducing, into the host cell cultured according to step (c), a detectably labeled trimethoprim; and

    (e) detecting the presence of the target protein by detecting labeled trimethoprim bound to the fusion protein.

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