Method of screening an antibody for activity in clearing an amyloid deposit
First Claim
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1. A method of screening a monoclonal antibody for activity in inducing clearance of an amyloid deposit of Aβ
- , comprising;
combining the amyloid deposit, a monoclonal antibody which binds to an epitope within amino acid residues 1-7 of Aβ
, and microglial cells bearing Fc receptors in a medium in vitro; and
by a series of measurements, monitoring whether a reduction in the amount of the amyloid deposit remaining in the medium occurs, as compared to a baseline measurement, a reduction in the amount of the amyloid deposit indicating the monoclonal antibody induces phagocytic clearing activity of the microglial cells against the amyloid deposit.
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Abstract
The invention provides improved agents and methods for treatment of diseases associated with amyloid deposits of Aβ in the brain of a patient. Such methods entail administering agents that induce a beneficial immunogenic response against the amyloid deposit. The methods are useful for prophylactic and therapeutic treatment of Alzheimer'"'"'s disease. Preferred agents including N-terminal fragments of Aβ and antibodies binding to the same.
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4 Claims
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1. A method of screening a monoclonal antibody for activity in inducing clearance of an amyloid deposit of Aβ
- , comprising;
combining the amyloid deposit, a monoclonal antibody which binds to an epitope within amino acid residues 1-7 of Aβ
, and microglial cells bearing Fc receptors in a medium in vitro; andby a series of measurements, monitoring whether a reduction in the amount of the amyloid deposit remaining in the medium occurs, as compared to a baseline measurement, a reduction in the amount of the amyloid deposit indicating the monoclonal antibody induces phagocytic clearing activity of the microglial cells against the amyloid deposit. - View Dependent Claims (2)
- , comprising;
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3. A method of screening a monoclonal antibody for activity in inducing clearance of an amyloid deposit of Aβ
- , comprising;
combining the amyloid deposit, a monoclonal antibody, and microglial cells bearing Fc receptors in a medium in vitro, wherein the amyloid deposit is a tissue sample from the brain of an Alzheimer'"'"'s disease patient or an animal having Alzheimer'"'"'s pathology; and by a series of measurements, monitoring whether a reduction in the amount of the amyloid deposit remaining in the medium occurs, as compared to a baseline measurement, a reduction in the amount of the amyloid deposit indicating the monoclonal antibody induces phagocytic clearing activity of the microglial cells against the amyloid deposit. - View Dependent Claims (4)
- , comprising;
Specification