Alcohol product processes

• US 7,579,177 B2
• Filed: 05/28/2004
• Issued: 08/25/2009
• Est. Priority Date: 05/30/2003
• Status: Active Grant

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Wumpelmann, Mogens, Tams, Jeppe Wegener, Norman, Barrie Edmund, Olsen, Hans Sejr
• Primary Examiner(s): Lilling; Herbert J.
• Application Number:
  US10/558,552
• Publication Number:
  US 20070031952A1
• Time in Patent Office: 1,915 Days
• Field of Search: 435/161, 426/11
• US Class Current: 435/162
• CPC Class Codes: C12N 9/2417 from microbiological source
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention relates to processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of said granular starch followed by simultaneous saccharification and fermentation.

Claims

1. A process for production of an alcohol product comprising the steps of:

(a) holding a slurry comprising water and granular starch at a temperature of 0°

C. to 20°

C below the initial gelatinization temperature of said granular starch for a period of 5 minutes to 12 hours,

(b) fermenting the slurry of step (a) with a yeast at a temperature between 10°

C. and 35°

C. for a period of 20 to 250 hours to produce ethanol,

wherein steps (a) and (b) are performed in the presence of an acid alpha-amylase activity, a maltose generating enzyme activity and an alpha-glucosidase activity.

2. The process according to claim 1, comprising recovering the ethanol.

3. The process according to claim 1, wherein the product is an ethanol product selected from the group consisting of fuel ethanol, potable ethanol andindustrial ethanol.

4. The process according to claim 1, wherein the temperature under step (b) is between 29°

C. and 35°

C.

5. The process according to claim 1, wherein the alcohol product is a beer.

6. The process according to claim 1, wherein the temperature under step (b) is between 11°

C. and 17°

C.

7. The process according to claim 1, wherein the acid alpha-amylase is an acid fungal alpha-amylase.

8. The process according to claim 1, wherein the acid fungal alpha-amylase is obtained from a strain of Aspergillus.

9. The process according to claim 1, wherein the acid fungal alpha-amylase is obtained from a strain of Aspergillus niger.

10. The process according to claim 1, wherein the acid alpha-amylase is an acid bacterial alpha-amylase.

11. The process according to claim 1, wherein the acid alpha-amylase is derived from a strain of B. licheniformis, B. amyloliquefaciens, or B. stearothermophilus alpha-amylase.

12. The process according to claim 1, wherein the acid bacterial alpha-amylase is derived from a strain of B. licheniformis, B. amyloliquefaciens, or B. stearothermophilus alpha-amylase.

13. The process according to claim 1, wherein the maltose generating enzyme activity is a maltogenic alpha-amylase.

14. The process according to claim 1, wherein the maltose generating enzyme activity is a beta-amylase.

15. The process according to claim 1, wherein the alpha-glucosidase activity is derived from a strain of Candida sp.

16. The process according to claim 1, wherein the alpha-glucosidase activity is derived from a strain of Bacillus sp.

17. The process according to claim 1, wherein step (a) and (b) are performed in the presence of a pullulanase.

18. The process according to claim 1, wherein step (a) or step (b) is performed in the presence of an enzyme activity selected from the group consisting of xylanase, cellulase and phytase.

19. The process of claim 1, wherein the starch slurry has 5-60% DS granular starch.

20. The process of claim 1, wherein the pH during step (a) or (b) is in the range of 3.0 to 7.0.

21. The process of claim 1, wherein the granular starch is obtained from tubers, roots, stems, fruits, seeds or whole grain, or cereal, corn, cobs, wheat, barley, rye, milo, sago, cassava, man ioc, tapioca, sorghum, rice or potatoes.

22. The process of claim 1, wherein the granular starch is obtained from dry milling or wet milling of whole grain.

23. The process according to claim 1, wherein the temperature under step (a) is from 45°

C. to 75°

C.

24. A process for production of an alcohol product comprising the steps of:

(a) holding a slurry comprising water and granular starch at a temperature of 0°

C. to 20°

C. below the initial gelatinization temperature of said granular starch,

(b) fermenting the slurry of step (a) with a yeast at a temperature between 10°

C. and 35°

C.,

wherein steps (a) and (b) are performed in the presence of an acid alpha-amylase activity, a maltose generating enzyme activity and an alpha-glucosidase activity.