Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
First Claim
1. A method for producing glial cells from human embryonic stem (hES) cells, comprisingculturing hES cells in suspension in a culture medium comprising basic fibroblast growth factor (bFGF), thyroid hormone T3, and retinoic acid (RA).
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Abstract
This invention provides populations of neural cells bearing markers of glial cells, such as oligodendrocytes and their precursors. The populations are generated by differentiating pluripotent stem cells such as human embryonic stem cells under conditions that promote enrichment of cells with the desired phenotype or functional capability. Various combinations of differentiation factors and mitogens can be used to produce cell populations bearing markers of oligodendrocyte precursor cells. Upon further differentiation form complex processes characteristic of mature oligodendrocytes. The cells are capable of forming myelin sheaths, and can be used therapeutically improve function of the central nervous system.
26 Citations
22 Claims
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1. A method for producing glial cells from human embryonic stem (hES) cells, comprising
culturing hES cells in suspension in a culture medium comprising basic fibroblast growth factor (bFGF), thyroid hormone T3, and retinoic acid (RA).
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15. A method for producing glial cells from human embryonic stem (hES) cells, comprising
a) culturing the hES cells in suspension in a culture medium comprising basic fibroblast growth factor (bFGF), and thyroid hormone T3; -
b) adding retinoic acid (RA) to the culture medium and continuing said culturing; c) replacing the culture medium with a replacement culture medium comprising thyroid hormone T3 and RA, wherein the replacement culture medium does not contain bFGF and culturing cells in the replacement culture medium; d) plating cells on an adherent surface; and e) culturing the plated cells in a culture medium comprising epidermal growth factor (EGF) and thyroid hormone T3; and f) harvesting cells that adhere to the surface. - View Dependent Claims (16, 17, 18, 19)
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20. A method for producing glial cells from human embryonic stem (hES) cells, comprising
a) culturing the hES cells in suspension in a culture medium comprising basic fibroblast growth factor (bFGF), thyroid hormone T3, and retinoic acid (RA); - and
b) replacing the culture medium with a replacement culture medium comprising thyroid hormone T3 and RA, wherein the replacement culture medium does not contain bFGF and culturing cells in the replacement culture medium; c) plating cells on an adherent surface; and d) culturing the plated cells in a culture medium comprising epidermal growth factor (EGF) and thyroid hormone T3; and e) harvesting cells that adhere to the surface; wherein the cells are cultured in the presence of RA for 8 days. - View Dependent Claims (21, 22)
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Specification