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Antisense antiviral compound and method for treating arenavirus infection

  • US 7,582,615 B2
  • Filed: 03/07/2007
  • Issued: 09/01/2009
  • Est. Priority Date: 03/07/2006
  • Status: Active Grant
First Claim
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1. A method of inhibiting viral infection in mammalian cells by an Arenavirus in the Arenaviridae family, comprising(a) exposing the cells to an antisense oligonucleotide compound composed of morpholino subunits and phosphorus-containing intersubunit linkages joining a morpholino nitrogen of one subunit to a 5′

  • exocyclic carbon of an adjacent subunit, and characterized by;

    (i) a substantially uncharged, nuclease-resistant backbone,(ii) capable of uptake by mammalian host cells,(iii) containing between 12-40 nucleotide bases,(iv) having a targeting sequence of at least 12 contiguous subunits complementary to SEQ ID NO;

    1, and(v) conjugated to the oligonucleotide, an arginine-rich polypeptide effective to promote uptake of the compound into infected host cells, and(b) by said exposing, forming a heteroduplex structure (i) composed of the virus'"'"' vRNA or vcRNA strand and the oligonucleotide compound, and (ii) characterized by a Tm of dissociation of at least 45°

    C.

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