Methods and compositions for treating mammalian spinal cord injuries

• US 7,582,680 B1
• Filed: 11/12/1999
• Issued: 09/01/2009
• Est. Priority Date: 11/12/1998
• Status: Expired due to Fees

• Current Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Original Assignee: National Science Foundation (Government of the United States of America), Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University)), The United States Of America As Represented By The Secretary Of The Army
• Inventors: Borgens, Richard B., Shi, Riyi
• Primary Examiner(s): HUI, SAN MING R
• Application Number:
  US09/438,206
• Time in Patent Office: 3,581 Days
• Field of Search: 514/723, 514/352
• US Class Current: 514/723
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/08 acyclic, e.g. paraformaldehyde
• CPC Class Codes: A61K 31/44 Non condensed pyridines; Hydrogenated derivatives thereof
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61L 2430/38 for reconstruction of the spine, vertebrae or intervertebral discs
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C08L 71/02 Polyalkylene oxides

Abstract

Methods of treating an injured vertebrate spinal cord are described. In one aspect of the invention, a method of treating an injured vertebrate spinal cord includes contacting the spinal cord with a biomembrane fusion agent such as a polyalkylene glycol, especially polyethylene glycol. In alternative embodiments of the invention, methods of treating an injured vertebrate spinal cord include contacting the cord with a biomembrane fusion agent and a potassium channel blocker. Other aspects of the invention include compositions for treating a vertebrate nervous system. A preferred composition includes a biomembrane fusion agent, such as a polyalkylene glycol, and a potassium channel blocker, such as an amino-substituted pyridine.

Claims

1. A method of treating a mammalian patient having suffered an injury to its spinal cord, said method comprising contacting the injured spinal cord after the injury but within a period no greater than about 24 hours after said injury with a composition comprising an effective amount of at least one C1-C10 polyalkylene glycol, wherein the effective amount of at least one C1-C10 polyalkylene glycol is effective to restore nerve impulse conduction through said injured spinal cord, and wherein the effective amount of at least one C1-C10 polyalkylene glycol is at least about 40% by weight in the composition.

2. The method according to claim 1 wherein said spinal cord is severed.

3. The method according to claim 1 wherein said spinal cord is crushed spinal cord.

4. The method according to claim 1 wherein said polyalkylene glycol is selected from the group consisting of polymethylene glycol, polyethylene glycol, polypropylene glycol, polybutylene glycol, polypentylene glycol, polyhexylene glycol, polyheptylene glycol, polyoctylene glycol, polynonylene glycol, polydecylene glycol and mixtures, thereof.

5. The method according to claim 4 wherein said polyalkylene glycol is administered to said patient in a pharmaceutically acceptable carrier.

6. The method according to claim 5 wherein said polyalkylene glycol is selected from the group consisting of polyethylene glycol, polypropylene glycol and mixtures thereof.

7. The method according to claim 1 wherein said polyalkylene glycol is polyethylene glycol.

8. The method according to claim 5 wherein said polyalkylene glycol is polyethylene glycol having a molecular weight ranging from about 40 daltons to about 3500 daltons.

9. The method according to claim 1, wherein said polyalkylene glycol is polyethylene glycol and wherein said method further comprises the step of contacting said injured spinal cord with a synergistic amount of 4-aminopyridine and within an effective time of contacting said spinal cord with said polyethylene glycol so as to produce a synergistic increase in restoration of nerve function and reflex behavior in said patient.

14. The method according to claim 1 or 10 wherein the restoration of nerve impulse conduction is evidenced by a detectable increase in conduction action potentials, observation of anatomical continuity, restoration of more than one spinal root level, or an increase in reflex behavior.

10. A method of treating a mammalian patient having suffered an injury to its spinal cord, said method comprising contacting the injured spinal cord after the injury but within a period no greater than about 24 hours after said injury with a composition comprising an effective amount of polyethylene glycol, wherein the effective amount of polyethylene glycol is effective to restore nerve impulse conduction through said injured spinal cord and wherein the effective amount of polyethylene glycol is at least about 40% by weight in the composition.

11. The method according to claim 10 wherein said polyethylene glycol has a molecular weight ranging from about 40 daltons to about 3500 daltons.

12. The method according to claim 10 further comprising the step of contacting said injured spinal cord with a potassium channel blocker in the form of 4-aminopyridine in an effective amount and within an effective time of contacting said spinal cord with said polyethylene glycol.

13. The method according to claim 12 wherein said polyethylene glycol has a molecular weight ranging from about 40 daltons to about 3500 daltons.