Reagent sets and gene signatures for renal tubule injury
First Claim
1. A method for testing whether a compound will induce renal tubule injury in a test subject, the method comprising:
- a) administering a dose of compound to at least one test subject, wherein the test subject is a mouse or rat;
b) after a selected time period, obtaining a biological sample from the at least one test subject;
c) measuring the expression levels in the biological sample of at least a plurality of sequences selected from Table 4, wherein the plurality of sequences comprises AI105417, BF404557, U08257, BF285022, and AF155910 and has at least 2% of the total impact of all of the sequences in Table 4; and
d) determining whether the sample is in the positive class for renal tubule injury using a classifier comprising at least the plurality of sequences for which the expression levels are measured.
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Abstract
The invention discloses reagent sets and gene signatures for predicting onset of renal tubule injury in a subject. The invention also provides a necessary set of 186 genes useful for generating signatures of varying size and performance capable of predicting onset of renal tubule injury. The invention also provides methods, apparatuses and reagents useful for predicting future renal tubule injury based on expression levels of genes in the signatures. In one particular embodiment the invention provides a method for predict whether a compound will induce renal tubule injury using gene expression data from sub-acute treatments.
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Citations
12 Claims
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1. A method for testing whether a compound will induce renal tubule injury in a test subject, the method comprising:
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a) administering a dose of compound to at least one test subject, wherein the test subject is a mouse or rat; b) after a selected time period, obtaining a biological sample from the at least one test subject; c) measuring the expression levels in the biological sample of at least a plurality of sequences selected from Table 4, wherein the plurality of sequences comprises AI105417, BF404557, U08257, BF285022, and AF155910 and has at least 2% of the total impact of all of the sequences in Table 4; and d) determining whether the sample is in the positive class for renal tubule injury using a classifier comprising at least the plurality of sequences for which the expression levels are measured. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification