Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism
First Claim
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1. A process for producing a transgenic plant comprisinga) transforming a cell or tissue of a plant with a nucleic acid encoding a polypeptide having the following three histidine-rich regions (i), (ii) and (iii):
- (i) His-(Xaa)3-His (SEQ ID NO;
21) orHis-(Xaa)4-His (SEQ ID NO;
22);
(ii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24); and
(iii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24),wherein His designates histidine, Xaa designates any naturally-occurring amino acid, (Xaa)3 refers to a sequence of three amino acids, (xaa)4 refers to a sequence of four amino acids, and (Xaa)2 refers to a sequence of two amino acids,wherein the polypeptide comprises a sequence of amino acids at least 65% identical to the sequence of amino acids set forth in SEQ ID NO;
2,wherein the nucleic acid encodes an expoxygenase, andwherein the nucleic acid is under the control of a promoter conferring transcription of the nucleic acid in the plant;
b) regenerating the transformed cell or tissue to produce the transgenic plant; and
c) examining the transgenic plant or part thereof for the presence of epoxy fatty acids to determine whether the transgenic plant has epoxy fatty acids.
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Abstract
The present invention relates generally to novel genetic sequences that encode fatty acid epoxygenase enzymes, in particular fatty acid Δ12-epoxygenase enzymes from plants that are mixed function monooxygenase enzymes. More particularly, the present invention exemplifies cDNA sequences from Crepis spp. and Vernonia galamensis that encode fatty acid Δ12-epoxygenases. The genetic sequences of the present invention provide the means by which fatty acid metabolism may be altered or manipulated in organisms, such as, for example, yeasts, moulds, bacteria, insects, birds, mammals and plants, and more particularly in plants. The invention also extends to genetically modified oil-accumulating organisms transformed with the subject genetic sequences and to the oils derived therefrom. The oils thus produced provide the means for the cost-effective raw materials for use in the efficient production of coatings, resins, glues, plastics, surfactants and lubricants.
74 Citations
17 Claims
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1. A process for producing a transgenic plant comprising
a) transforming a cell or tissue of a plant with a nucleic acid encoding a polypeptide having the following three histidine-rich regions (i), (ii) and (iii): -
(i) His-(Xaa)3-His (SEQ ID NO;
21) orHis-(Xaa)4-His (SEQ ID NO;
22);(ii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24); and(iii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24),wherein His designates histidine, Xaa designates any naturally-occurring amino acid, (Xaa)3 refers to a sequence of three amino acids, (xaa)4 refers to a sequence of four amino acids, and (Xaa)2 refers to a sequence of two amino acids, wherein the polypeptide comprises a sequence of amino acids at least 65% identical to the sequence of amino acids set forth in SEQ ID NO;
2,wherein the nucleic acid encodes an expoxygenase, and wherein the nucleic acid is under the control of a promoter conferring transcription of the nucleic acid in the plant; b) regenerating the transformed cell or tissue to produce the transgenic plant; and c) examining the transgenic plant or part thereof for the presence of epoxy fatty acids to determine whether the transgenic plant has epoxy fatty acids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 16, 17)
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11. A process for producing a transformed plant cell comprising introducing into the plant cell a nucleic acid encoding a polypeptide having the following three histidine-rich regions (i), (ii) and (iii):
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(i) His-(Xaa)3—
His (SEQ ID NO;
21) orHis-(Xaa)4—
His (SEQ ID NO;
22);(ii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24); and(iii) His-(Xaa)2-His-His (SEQ ID NO;
23) orHis-(Xaa)3-His-His (SEQ ID NO;
24),wherein His designates histidine, Xaa designates any naturally-occurring amino acid, (xaa)3 refers to a sequence of three amino acids, (Xaa)4 refers to a sequence of four amino acids, and (Xaa)2 refers to a sequence of two amino acids, wherein the polypeptide comprises a sequence of amino acids at least 65% identical to the amino acid sequence set forth in SEQ ID NO;
2,wherein the nucleic acid encodes an expoxygenase, and wherein the nucleic acid is under the control of a promoter conferring transcription of the nucleic acid in a plant cell and is stably integrated into the genome of the cell, thereby producing the transformed plant cell. - View Dependent Claims (12, 13, 14, 15)
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Specification
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Current AssigneeBASF Plant Science GmbH (BASF SE), Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
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Original AssigneeCommonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
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InventorsStymne, Sten, Green, Allan, Singh, Surinder, Lenman, Marit
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Primary Examiner(s)McElwain; Elizabeth F
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Application NumberUS09/981,124Publication NumberTime in Patent Office2,890 DaysField of Search800/298, 800/281, 800/306, 800/317, 800/314, 800/322, 435/416, 435/468US Class Current800/281CPC Class CodesC12N 15/8247 involving modified lipid me...C12N 9/0004 Oxidoreductases (1.)C12N 9/0071 acting on paired donors wit...C12P 7/6409 Fatty acidsC12P 7/6427 Polyunsaturated fatty acids...C12P 7/6472 containing polyunsaturated ...
