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Method of forming mesenchymal stem cells from embryonic stem cells

  • US 7,592,176 B2
  • Filed: 05/06/2005
  • Issued: 09/22/2009
  • Est. Priority Date: 05/07/2004
  • Status: Active Grant
First Claim
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1. A method of producing a population of human mesenchymal lineage cells comprising mesenchymal stem cells (MSCs) and bone precursor cells (BPCs) from human embryonic stem cells (hESCs), the method comprising the steps of:

  • a) culturing hESCs under conditions conducive to form embryoid bodies (EBs);

    b) propagating the EBs formed in step a) in a mesenchyme-specific medium containing 1.25(OH)2D3 to promote formation of EBs having cells of the mesenchymal lineage;

    c) digesting the resultant EBs of step b) and culturing the resulting cells in mesenchyme-specific medium to obtain a population of human mesenchymal lineage cells exhibiting spindle shaped morphology, wherein the population is composed of at least 60% human MSCs, and wherein the population exhibits enhanced expression of Cba-1, Msx2, and D1x5 relative to the EBs of step a); and

    d) propagating the human MSCs produced by step c).

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