Apparatus and method for measuring activity signals of biological samples

• US 7,594,984 B2
• Filed: 01/12/2007
• Issued: 09/29/2009
• Est. Priority Date: 05/13/2002
• Status: Active Grant

• Current Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Oka, Hiroaki, Ozaki, Nobuhiko, Sugihara, Hirokazu
• Primary Examiner(s): Lilling; Herbert J.
• Application Number:
  US11/652,605
• Publication Number:
  US 20070134651A1
• Time in Patent Office: 991 Days
• Field of Search: None
• US Class Current: 204/403.1
• CPC Class Codes: G01N 33/48728 Investigating individual cells, e.g. by patch clamp, voltage clamp investigating individual particles in general G01N15/10
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

Claims

1. An apparatus for measuring activity signals of a biological sample comprising:

a measurement chamber storing a target liquid containing a biological sample;

a porous insulating substrate provided with a measurement electrode on at least one side, said measurement electrode arranged on a surface of the porous insulating substrate; and

a conveying device which conveys the target liquid stored in the measurement chamber and passes the target liquid through the porous insulating substrate from the measurement electrode side,

wherein the conveying device is operated to trap the biological samples contained in the target liquid onto the measurement electrode, so that the activity signals of the biological samples are measured through the measurement electrode.

2. An apparatus for measuring activity signals of a biological sample according to claim 1, further comprising a reference electrode that is provided in the measurement chamber.

3. An apparatus for measuring activity signals of a biological sample according to claim 1, wherein the conveying device further comprises a suction apparatus for sucking and then passing the target liquid through the porous insulating substrate.

4. An apparatus for measuring activity signals of a biological sample according to claim 3, wherein the porous insulating substrate is detachably provided between the measurement chamber and the suction apparatus.

5. An apparatus for measuring activity signals of a biological sample according to claim 1, wherein the porous insulating substrate is composed of a resin film having a pore diameter of not more than 1 μ

m but not less than 1,000 μ

m and a thickness of not more than 1 μ

m but not less than 10,000 μ

m.

6. An apparatus for measuring activity signals of a biological sample according to claim 1, further comprising:

a conductor electrically connected to the measurement electrode, wherein the conductor is formed on one side of the porous insulating substrate via a mask layer and the measurement electrode is infused into the porous insulating substrate more deeply than the conductor.

7. An apparatus for measuring activity signals of a biological sample according to claim 6, wherein the measurement electrode and the conductor are formed by sputtering a conductive material onto a surface of the porous insulating substrate.

8. An apparatus for measuring activity signals of a biological sample according to claim 1, wherein the measurement chamber and the conductor are provided in plural;

the conveying device is provided with a common suction line communicating with each of the measurement chambers; and

the conveying device is operated to simultaneously trap the biological samples contained in the target liquid stored in the measurement chambers onto the corresponding measurement electrodes.

9. An apparatus for measuring activity signals of a biological sample according to claim 8, further comprising a plurality of reference electrodes provided in the plurality of measurement chambers, respectively.

10. An apparatus for measuring activity signals of a biological sample according to claim 1, further comprising:

a means for calculating the standard deviation per a predetermined number of samples based on time-series data of activity signals outputted via the measurement electrode;

a means for calculating the mean value of a plurality of the obtained standard deviations;

a means for evaluating the activity of the biological samples based on the obtained mean value of the standard deviations; and

a means for displaying the results of the activity evaluation.

11. An apparatus for measuring activity signals of a biological sample according to claim 1, further comprising:

a means for calculating the standard deviation per a predetermined number of samples based on time-series data of activity signals outputted via the measurement electrode;

a means for classifying the obtained plurality of the standard deviations into a plurality of classes set by a predetermined width and approximating the classification results to the normal distribution;

a means for evaluating the activity of the biological samples based on the obtained normal distribution; and

a means for displaying the results of the activity evaluation.

12. An apparatus for measuring activity signals of a biological sample according to claim 11, further comprising:

a means for calculating the mean value and half-width of the obtained normal distribution, wherein the activity is evaluated based on the obtained mean value and half-width of the normal distribution.

13. An apparatus for measuring activity signals of a biological sample according to claim 1, wherein said porous insulating substrate includes another surface opposite said surface, said another surface facing away from the measurement chamber.

14. An apparatus for measuring activity signals of a biological sample according to claim 1, wherein the measurement electrode contacts the porous insulating substrate.