Automated sample analysis
First Claim
1. A method of determining unknown concentrations of an inorganic ion of interest in a recovered sample comprising the steps of:
- providing a microfluidic device comprising a sample well having the recovered sample disposed therein, an indicator well having a fluorescent indicator disposed therein, a control well having a control disposed therein, the control including a known quantity of the inorganic ion of interest, a waste well, a detection region, and a light source directed at the detection region;
wherein the sample well, the indicator well, and the waste well form a first fluidic circuit, the sample well fluidly coupled to the waste well via a first channel, and the indicator well fluidly connected to the first channel via a second channel;
and wherein the indicator well, the control well, and the waste well form a second fluidic circuit, the indicator well fluidly coupled to the waste well via a third channel, and the control well fluidly coupled to the third channel via fourth, fifth, and sixth channels, each of the fourth, fifth, and sixth channels having a different flow resistance;
mixing, in the third channel, the control sample and the fluorescent indicator, wherein flowing the control sample into the third channel via the fourth, fifth, and sixth channels provides a plurality of relative known ratios of the control sample in the indicator;
obtaining a signal representative of the concentration of the inorganic ion of interest in each of the ratios, wherein the signal comprises an intensity of fluorescence;
generating a model representative of the signal and the relative ratio of the control sample in the indicator;
mixing, in the first channel, the recovered sample and the fluorescent indicator, and obtaining a signal representative of the concentration of the inorganic ion of interest therein, wherein the signal comprises an intensity of fluorescence; and
comparing the signal representative of the inorganic ion of interest for the recovered sample within the model, thereby providing a determination of the concentration of the inorganic ion of interest present in the recovered sample.
1 Assignment
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Accused Products
Abstract
Samples of materials used in industrial processes are analyzed to determine the concentration of certain materials of interest. The quantitative analysis of samples for these materials is provided without the need for manual methods such as titration. Indicators such as fluorescent dyes for which the intensity of fluorescence is indicative of the concentration of a material of interest are used. The dyes are made to fluoresce by means of a light source, and a photomultiplier or other detector capable of measuring light intensity detects the resulting fluorescence. The intensity of fluorescence in the sample is compared to the intensities of fluorescence produced by samples with known concentrations of the material of interest to determine the concentration of the material of interest of the sample.
91 Citations
7 Claims
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1. A method of determining unknown concentrations of an inorganic ion of interest in a recovered sample comprising the steps of:
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providing a microfluidic device comprising a sample well having the recovered sample disposed therein, an indicator well having a fluorescent indicator disposed therein, a control well having a control disposed therein, the control including a known quantity of the inorganic ion of interest, a waste well, a detection region, and a light source directed at the detection region; wherein the sample well, the indicator well, and the waste well form a first fluidic circuit, the sample well fluidly coupled to the waste well via a first channel, and the indicator well fluidly connected to the first channel via a second channel; and wherein the indicator well, the control well, and the waste well form a second fluidic circuit, the indicator well fluidly coupled to the waste well via a third channel, and the control well fluidly coupled to the third channel via fourth, fifth, and sixth channels, each of the fourth, fifth, and sixth channels having a different flow resistance; mixing, in the third channel, the control sample and the fluorescent indicator, wherein flowing the control sample into the third channel via the fourth, fifth, and sixth channels provides a plurality of relative known ratios of the control sample in the indicator; obtaining a signal representative of the concentration of the inorganic ion of interest in each of the ratios, wherein the signal comprises an intensity of fluorescence; generating a model representative of the signal and the relative ratio of the control sample in the indicator; mixing, in the first channel, the recovered sample and the fluorescent indicator, and obtaining a signal representative of the concentration of the inorganic ion of interest therein, wherein the signal comprises an intensity of fluorescence; and comparing the signal representative of the inorganic ion of interest for the recovered sample within the model, thereby providing a determination of the concentration of the inorganic ion of interest present in the recovered sample. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification