Spectroscopic methods for component particle analysis

• US 7,595,878 B2
• Filed: 03/28/2005
• Issued: 09/29/2009
• Est. Priority Date: 10/13/2000
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Attanucci, Jason, Treado, Patrick, Nelson, Matthew P.
• Primary Examiner(s): Toatley, Jr.; Gregory J.
• Assistant Examiner(s): GIGLIO, BRYAN J
• Application Number:
  US11/091,126
• Publication Number:
  US 20060019409A1
• Time in Patent Office: 1,646 Days
• Field of Search: 356/335, 356/601, 356/612
• US Class Current: 356/335
• CPC Class Codes: G06V 20/69 Microscopic objects, e.g. biological cells or cellular parts

Abstract

The invention relates to methods of assessing one or more geometric properties of a particle of a substance using an infrared spectroscopic property of the substance. The method is useful, for example, for assessing particle sizes and size distributions in mixtures containing both particles of the substance and other materials.

Claims

1. A method of determining a geometric property of a particle of a substance in a sample, the method comprising:

irradiating the sample;

tuning an imaging spectrometer over a range of wavelengths to generate an infrared image of the sample at a wavelength characteristic of the substance;

applying to the infrared image of the sample one or more of the following;

a univariate image processing technique, and a multivariate image processing technique to thereby generate a two-dimensional image or a three-dimensional image;

measuring the geometric property of the particle of the substance in the sample using the two-dimensional image or the three-dimensional image to thereby generate a numerical value for the geometric property;

and reporting said numerical value of the geometric property of the particle.

2. The method of claim 1, wherein the infrared image is a near infrared image.

3. The method of claim 1, wherein the infrared image is an image of a microscopic field.

4. The method of claim 1, further comprising simultaneously determining the geometric property of multiple particles of the substance in the sample.

5. The method of claim 1, wherein the geometric property is characteristic of the size of the particle.

6. The method of claim 1, comprising generating an infrared image of the sample at multiple infrared wavelengths characteristic of the substance.

7. The method of claim 1, further comprising comparing a first image and a second image of the sample at a wavelength characteristic of a compound other than the substance.

8. The method of claim 7, wherein the second image is a near infrared image.

9. The method of claim 7, wherein the first and second images are combined in an aligned manner.

10. The method of claim 7, comprising determining the geometric properties of the particle of the substance and of a particle of the compound in the sample.

11. The method of claim 1, wherein the infrared image is the two-dimensional image.

12. The method of claim 11, wherein the geometric property is selected from the group consisting of the area, the perimeter, a Feret diameter, the maximum chord length, a shape factor, and an aspect ratio of the particle.

13. The method of claim 1, wherein the infrared image is the three-dimensional image.

14. The method of claim 13, wherein the geometric property is selected from the group consisting of the volume, the surface area, a Feret diameter, the maximum chord length, a shape factor, and an aspect ratio of the particle.

15. The method of claim 1, wherein the particle is irradiated with substantially monochromatic light.

16. The method of claim 1, wherein the particle is immobilized prior to generating the image.

17. A method of determining a geometric property of a particle of a substance in a sample, the method comprising irradiating the sample, generating a near infrared image of the sample at a wavelength characteristic of the substance, wherein the near infrared image is generated using a near infrared radiation chemical imaging system comprising:

a) an illumination source for illuminating an area of the sample using light in the near infrared radiation wavelength;

b) a device for collecting a spectrum of near infrared wavelength radiation light transmitted from the illuminated area in a focal plane and producing a collimated beam therefrom;

c) a near infrared imaging spectrometer for filtering a near infrared radiation image of the collimated beam; and

d) a detector for collecting the filtered near infrared image and generating the near infrared image;

applying to the infrared image of the sample one or more of the following;

a univariate image processing technique, and a multivariate image processing technique to thereby generate a two-dimensional image or a three-dimensional image;

measuring the geometric property of the particle of the substance in the sample using the two-dimensional image or the three-dimensional image to thereby generate a numerical value for the geometric property; and

reporting said numerical value of the geometric property of the particle.

18. A method of determining geometric properties of multiple particles of a substance in a sample, the method comprising:

irradiating the sample;

tuning an imaging spectrometer over a range of wavelengths to generate an infrared image of the sample at a wavelength characteristic of the substance;

identifying the multiple particles of the substance by assessing the infrared image at the wavelength characteristic of the substance;

generating an optical image of the multiple particles in the substance, wherein the optical image is generated by a spectroscopic method other than infrared spectroscopy;

measuring the geometric properties of the multiple particles in the substance using the optical image; and

reporting the geometric properties of the multiple particles.

19. A method of assessing geometric properties and compositions of a particle agglomerate comprised of first particles of a first substance and second particles of a second substance in a sample, the method comprising:

irradiating the sample;

tuning an imaging spectrometer over a range of wavelengths to generate a first infrared image of the sample at a first wavelength characteristic of the first substance and a second infrared image of the sample at a second wavelength characteristic of the second substance;

comparing the first infrared image with the second infrared image of the sample at said second wavelength characteristic of the second substance to differentiate the composition of the first particles of the first substance from the composition of the second particles of the second substance;

generating an optical image of the first particles of the first substance and the second particles of the second substance, wherein the optical image is generated by a spectroscopic method other than infrared spectroscopy

measuring the geometric properties of the first particles of the first substance and the second particles of the second substance in the sample using the optical image; and

reporting the composition and geometric properties of the first particles of the first substance and the composition and geometric properties of the second particles of the second substance.

20. The method of claim 19, further comprising:

identifying a composition of the particle agglomerate of the substance by assessing the first infrared image at the first wavelength characteristic of the first substance and the second infrared image at the second wavelength characteristic of the second substance;

generating an optical image of the particle agglomerate in the sample, wherein the optical image is generated by a spectroscopic method other than infrared spectroscopy;

measuring the geometric properties of the particle agglomerate in the substance using the optical image; and

reporting the geometric property of the particle agglomerate.

21. A method of determining a geometric property of a particle of a substance in a sample, the method comprising:

irradiating the sample;

generating a near infrared image of the sample at a wavelength characteristic of the substance, wherein the near infrared image is generated using a near infrared radiation chemical imaging system comprising;

a) an illumination source for illuminating an area of the sample using light in the near infrared radiation wavelength;

b) a device for collecting a spectrum of near infrared wavelength radiation light reflected from the illuminated area in a focal plane and producing a collimated beam therefrom;

c) a near infrared imaging spectrometer for filtering a near infrared radiation image of the collimated beam; and

d) a detector for collecting the filtered near infrared image and generating a near infrared image;

applying to the infrared image of the sample one or more of the following;

a univariate image processing technique, and a multivariate image processing technique to thereby generate a two-dimensional image or a three-dimensional image;

measuring the geometric property of the particle of the substance in the sample using the two-dimensional image or the three-dimensional image to thereby generate a numerical value for the geometric property; and

reporting said numerical value of the geometric property of the particle.

22. A method of determining a geometric property of a particle of a substance in a sample, the method comprising:

irradiating the sample;

generating a near infrared image of the sample at a wavelength characteristic of the substance, wherein the near infrared image is generated using a near infrared radiation chemical imaging system comprising;

a) an illumination source for illuminating an area of the sample using light in the near infrared radiation wavelength;

b) a device for collecting a spectrum of near infrared wavelength radiation light emitted from the illuminated area in a focal plane and producing a collimated beam therefrom;

c) a near infrared imaging spectrometer for filtering a near infrared radiation image of the collimated beam; and

d) a detector for collecting the filtered near infrared image and generating a near infrared image;

applying to the infrared image of the sample one or more of the following;

a univariate image processing technique, and a multivariate image processing technique to thereby generate a two-dimensional image or a three-dimensional image;

measuring the geometric property of the particle of the substance in the sample using the two-dimensional image or the three-dimensional image to thereby generate a numerical value for the geometric property; and

reporting said numerical value of the geometric property of the particle.

23. A method of determining a geometric property of a particle of a substance in a sample, the method comprising:

irradiating the sample;

generating a near infrared image of the sample at a wavelength characteristic of the substance, wherein the near infrared image is generated using a near infrared radiation chemical imaging system comprising;

a) an illumination source for illuminating an area of the sample using light in the near infrared radiation wavelength;

b) a device for collecting a spectrum of near infrared wavelength radiation light elastically scattered from the illuminated area in a focal plane and producing a collimated beam therefrom;

c) a near infrared imaging spectrometer for filtering a near infrared radiation image of the collimated beam; and

d) a detector for collecting the filtered near infrared image and generating a near infrared image;

applying to the infrared image of the sample one or more of the following;

a univariate image processing technique, and a multivariate image processing technique to thereby generate a two-dimensional image or a three-dimensional image;

measuring the geometric property of the particle of the substance in the sample using the two-dimensional image or the three-dimensional image to thereby generate a numerical value for the geometric property; and

reporting said numerical value of the geometric property of the particle.